Evaluation of Direct Plating and Enrichment Methods for Isolation of <i>Vibrio cholerae</i> O139 from Faecal Samples

Authors

  • Mahbubur Rahman Laboratory Sciences Division, Centre for Health & Population Research, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), GPO Box 128, Mohakhali, Dhaka 1212, Bangladesh
  • Mostofa Kamal Laboratory Sciences Division, Centre for Health & Population Research, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), GPO Box 128, Mohakhali, Dhaka 1212, Bangladesh
  • Shereen Shoma Laboratory Sciences Division, Centre for Health & Population Research, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), GPO Box 128, Mohakhali, Dhaka 1212, Bangladesh
  • Manuel John Albert Laboratory Sciences Division, Centre for Health & Population Research, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), GPO Box 128, Mohakhali, Dhaka 1212, Bangladesh
  • Abul Kashem Siddique Public Health Sciences Division, Centre for Health & Population Research, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), GPO Box 128, Mohakhali, Dhaka 1212, Bangladesh

DOI:

https://doi.org/10.3329/bjm.v23i2.880

Keywords:

Cholera, Vibrio cholerae O139, enrichment cultures, direct-plate culture

Abstract

In this study, direct-plate culture method alone, or in combination with enrichment culture for isolation of Vibrio cholerae O139 from faecal samples at a Diarrhoea Treatment Centre in Dhaka, Bangladesh had been evaluated. Faecal samples of 528 patients with acute phase of diarrhoea, attended the Centre, were cultured directly onto thiosulfate-citrate-bile-salts-sucrose agar (direct-TCBSA) and tellurite-taurocholate-gelatin agar (direct-TTGA) and after 6-h and overnight enrichment in alkaline-bile-peptone-broth for V. cholerae O139 (ABPB) [6h-ABPB-TCBSA and 6h-ABPB-TTGA, and overnight-ABPB-TCBSA and overnight-ABPBTTGA]. Direct-TTGA-plating and overnight-ABPB-TTGA methods (latter for samples yielding negative results by overnight-direct-TTGA cultures) were also performed for another 18,647 faecal samples submitted for culture in our laboratory and 1,552 rectal swabs transported in Cary-Blair medium during investigation of acute diarrhoea outbreaks in the country. During acute phase of diarrhoea, direct-TCBSA, or direct-TTGA cultures equally supported O139 growth, while direct-plating plus 6-h, or overnight enrichment cultures were not superior as compared to the direct-plating. Direct-TTGA-plating plus overnight-ABPB-TTGA increased O139 isolation from transported rectal swabs. Thus, direct-plating provides optimum stool culture results for V. cholerae O139 during acute phase of diarrhoea. However, a combination of direct-TTGA-plating and overnight- ABPB-TTGA cultures was useful for isolation of V. cholerae O139 from transported rectal swab samples.

Keywords: Cholera, Vibrio cholerae O139, enrichment cultures, direct-plate culture

DOI: http://dx.doi.org/10.3329/bjm.v23i2.880

Bangladesh J Microbiol, Volume 23, Number 2, December 2006, pp 140-144

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How to Cite

Rahman, M., Kamal, M., Shoma, S., Albert, M. J., & Siddique, A. K. (2008). Evaluation of Direct Plating and Enrichment Methods for Isolation of <i>Vibrio cholerae</i> O139 from Faecal Samples. Bangladesh Journal of Microbiology, 23(2), 140–144. https://doi.org/10.3329/bjm.v23i2.880

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