Detection of Gamma Radiation-Induced Oxidation in Animal and Bacterial DNA

Authors

  • M Khorshed Alam Institute of Food & Radiation Biology (IFRB), Atomic Energy Research Establishment (AERE), Ganakbari, Savar, GPO Box 3787, Dhaka 1000, Bangladesh

DOI:

https://doi.org/10.3329/bjm.v23i2.877

Keywords:

Gamma radiation-induced oxidation, 8-Hydroxydeoxyguanine (8-OHdG), ELISA, CHO-K1, Bovine, Chicken, Escherichia coli, Deinococcus radiodurans

Abstract

Gamma radiation-induced oxidative DNA damage, in terms of production of 8-hydroxydeoxyguanine (8-OHdG), was studied in the DNA samples from Chinese hamster ovary cell line (CHO-K1), bovine, chicken, Escherichia coli and Deinococcus radiodurans. A radiation dose response with respect to the formation of the 8-OHdG residues from the DNA molecules was observed by employing enzyme-linked immunosorbent assay (ELISA) detection system using monoclonal antibody. At high radiation doses (>5 kGy), the formation or detection of the 8-OHdG residues from bacterial DNA was declined. It was apparent that the oxidative DNA damage was dependent on the base composition of the DNA molecule. However, an inverse relation was observed between the dose limit and nitrogenous base composition especially the guanine residue. Nonetheless, DNA base composition might to be considered in cases of the radiation-induced oxidation of food or other DNA samples.

Keywords: Gamma radiation-induced oxidation, 8-Hydroxydeoxyguanine (8-OHdG), ELISA, CHO-K1, Bovine, Chicken, Escherichia coli, Deinococcus radiodurans  

DOI: http://dx.doi.org/10.3329/bjm.v23i2.877

Bangladesh J Microbiol, Volume 23, Number 2, December 2006, pp 130-132

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Published

2008-06-09

How to Cite

Alam, M. K. (2008). Detection of Gamma Radiation-Induced Oxidation in Animal and Bacterial DNA. Bangladesh Journal of Microbiology, 23(2), 130–132. https://doi.org/10.3329/bjm.v23i2.877

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