Characterization of Arsenite Resistant Escherichia coli Isolated from Tubewell Water of Singair, Manikganj, Bangladesh

Authors

  • Santonu Kumar Sanyal Department of Microbiology, University of Dhaka, Dhaka 1000
  • Ram Prosad Chakrabarty Department of Microbiology, University of Dhaka, Dhaka 1000
  • M Anwar Hossain Department of Microbiology, University of Dhaka, Dhaka 1000
  • Munawar Sultana Department of Microbiology, University of Dhaka, Dhaka 1000

DOI:

https://doi.org/10.3329/bjm.v31i1.28459

Keywords:

Escherichia coli, arsB gene, Plasmid, Arsenite resistance

Abstract

Arsenic is a toxic metalloid present in the natural environment and arsenite is more toxic form than arsenate. Bacterial species have evolved multiple defense mechanisms to tolerate and transform the toxic forms of arsenic. In this study, arsenic contaminated tubewell water (90 meter depth with arsenic concentration of 47 ?g/l) was collected from Singair Upazilla under Manikganj District in Bangladesh. Among a total of 17 arsenite resistant bacteria, a lactose fermenting isolate Sn26 showed high resistance to sodium arsenite up to 25 mM under our laboratory conditions. Sn26 possesses arsB gene conferring arsenite transporter pump mediated arsenic resistance, confirmed by PCR and sequencing. PCR and sequencing of 16S rRNA gene (approximately 1450 bp) confirmed Sn26 isolate as Escherichia coli. The isolate contained a large plasmid of ~34 kb. It is necessary to analyze further the affiliation of this single plasmid with high arsenite tolerance.

Bangladesh J Microbiol, Volume 31, Number 1-2,June-Dec 2014, pp 13-17

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Author Biography

Santonu Kumar Sanyal, Department of Microbiology, University of Dhaka, Dhaka 1000



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Published

2016-06-25

How to Cite

Sanyal, S. K., Chakrabarty, R. P., Hossain, M. A., & Sultana, M. (2016). Characterization of Arsenite Resistant Escherichia coli Isolated from Tubewell Water of Singair, Manikganj, Bangladesh. Bangladesh Journal of Microbiology, 31(1), 13–17. https://doi.org/10.3329/bjm.v31i1.28459

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