Analysis of Antibiotics Susceptibility of Old and Fresh ATCC Strain of <i>Staphylococcus aureus</i> by Standard Agar Diffusion Technique

Authors

  • Marufa Zerin Akhter Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh
  • Ibrahim Khalil Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh
  • Priyanath Roy Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh
  • Mir Mohammad Ibna Masud Control Department, Beximco Pharmaceuticals Limited, Tongi 1711, Gazipur, Bangladesh

DOI:

https://doi.org/10.3329/bjm.v24i2.1259

Keywords:

Staphylococcus aureus, Antibiotic resistance mechanism

Abstract

A comparative study of an old and fresh ATCC strain of Staphylococcus aureus was carried out to determine the mechanism of antibiotic resistance of the old one, which had been preserved in lyophilized condition. Five different antibiotics having five different modes of action were used. They included amoxicillin, azithromycin, neomycin, ciprofloxacin and polymixin B. Agar diffusion technique was followed to determine the antibiotic sensitivity. It was observed that the old strain showed the highest resistance against amoxicillin, whereas no remarkable changes in the sensitivity pattern were observed against polymixin B and neomycin. In case of azithromycin and ciprofloxacin, the old strain showed resistance up to some extent. From these results it was clearly evident that in the controlled preserved conditions, cell wall-mediated resistance was predominating, while the cell membrane- and 30S ribosome-mediated resistance were the least likely ones. The nucleic acid synthesis inhibitors and the 50S ribosomal protein synthesis inhibitor lied in between these two extremes showing variations up to some extent in the sensitivity pattern between the old and fresh ATCC strain of S. aureus.

Keywords: Staphylococcus aureus, Antibiotic resistance mechanism

DOI: http://dx.doi.org/10.3329/bjm.v24i2.1259

Bangladesh J Microbiol, Volume 24, Number 2, December 2007, pp 137-142

Downloads

Download data is not yet available.
Abstract
1365
PDF
1025

Downloads

How to Cite

Akhter, M. Z., Khalil, I., Roy, P., & Masud, M. M. I. (2008). Analysis of Antibiotics Susceptibility of Old and Fresh ATCC Strain of <i>Staphylococcus aureus</i> by Standard Agar Diffusion Technique. Bangladesh Journal of Microbiology, 24(2), 137–142. https://doi.org/10.3329/bjm.v24i2.1259

Issue

Section

Articles