Isolation and molecular detection of respiratory bacterial agents from buffalo reared in some selected areas of Bangladesh
In buffalo respiratory bacterial infection is very common which occurs sporadically or enzootically all over Bangladesh causing high economic loss. The study was performed with a view to proper control of respiratory bacterial infection in Buffalo. A total of 40 samples were collected on the basis of clinical signs. The samples were then subjected to isolation, identification and characterization of the bacterial agents using cultural, biochemical and molecular techniques. Antibiogram profiles of the isolated agents were studied by disc diffusion method. Pasteurella multocida, Staphylococcus aureus and E. coli were successfully isolated and identified from the collected samples. The isolated Pasteurella multocida produced small, round, opaque colonies on blood agar; Staphylococcus aureus produced golden yellow colony in mannitol salt agar; E. coli produced black color colonies with metallic sheen on EMB agar. Pasteurella multocida showed Gram negative, bipolar rods. Staphylococcus aureus showed Gram positive, cocci shaped and E. coli showed Gram negative, small rod shaped. On the basis of cultural and biochemical characteristics, among 40 nasal samples 5 were found to be positive for Pasteurella multocida, 4 for Staphylococcus aureus and 3 for E. coli. Pasteurella multocida was further confirmed by PCR where isolates showed positive band at 620 bp. The antibiogram study concluded that amoxicillin, gentamicin, & ciprofloxacin should be the first choice of treatment for respiratory bacterial infection caused by the isolated 3 bacteria.
Asian J. Med. Biol. Res. December 2018, 4(4): 416-421
Copyright (c) 2018 Taslima Akter
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms / The author(s) affirm(s) that:
- The manuscript submitted is based on authors own research and is original work.
- Authors certify that we all participated in the research work and preparation of the manuscript in a substantive way.
- Authors also declare that they have read and approved the manuscript.
- Authors further declare that the manuscript has not been published in part or full and is not being submitted or considered for publication in part or full elsewhere.
- Any material included in the manuscript does not violate copyright or other rights of anyone.
- Authors also affirm that the article contains no vilifying or unlawful statements and does not contain material or instructions that might cause harm or injury to the Editor-in-Chief/Editors of the Journal and the public.
- Authors assure Asian J. Med. Biol. Res. and the Editor-in-Chief/Editors of the Journals, and hold them harmless from any loss, expense or damage occurred by a claim or suit by a third party for copyright violation, or any suit arising out of any violation of the foregoing
- In consideration of authors manuscript submitted, authors hereby grant Asian J. Med. Biol. Res. unlimited, worldwide, permanent royalty-free, right to publish, use, dispense, license, transmit, display, exhibit, record, store, translate, digitize, broadcast, reproduce and archive, in any format or medium, whether now known or developed hereafter.
- warranties as a result of publication of my/our article.
All materials related to manuscripts, accepted or rejected, including photographs, original figures etc., will be kept by Asian J. Med. Biol. Res. for one year following the editors decision. These materials will then be destroyed.