Isolation, identification and antibiogram profile of bacteria isolated from dental caries patients of Mymensingh district of Bangladesh

  • Shuvho Chakra Borty Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh- 2202
  • Khandakar Musabbir Bin Hafiz Khandakar Dental Clinic, Charpara, Mymensingh- 2202
  • Md Mortuza Ali Department of Community Medicine, Gono Shasthaya Samaj Vittik Medical College, Savar- 1344
  • Khadiza Begum Khandakar Dental Clinic, Charpara, Mymensingh- 2202
  • Tanvir Ahammed Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh- 2202
  • Md Shirajum Monir Bangladesh Fisheries Research Institute, Freshwater Station, Mymensingh-2201
  • Md Alimul Islam Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh- 2202
Keywords: dental caries, total viable count, bacteria, antibiotic sensitivity

Abstract

This study was carried out for the evaluation of causative agents of dental plaques which leads to dental caries. A total of 76 samples collected from the dental caries patients of various age and sex were processed on different bacterial isolation media like Tryptose Soya Agar, Nutrient Agar, and MacConkey Agar. Total viable count of bacteria was ranged from 0.8×105 to 2.9×105. Out of total 184 isolates bacterial species were Streptococcus mutans (28.80%), Streptococcus mitis (23.91%), Staphylococcus aureus (28.26%) and Streptococcus salivarius (19.02%). The biochemical properties of the isolates were tested according to Bergeys Manual of Systematic Bacteriology and UK Standards for Microbiology Investigation. Antibiotic Susceptibility testing of different species of bacteria were performed according to Kirby Bauer disc diffusion method on Muller Hinton Agar by using commercial antibiotic discs. Effect of the bacterial isolates on different age groups was studied where the age group of 26 to 35 years was found to have highest prevalence (32.60%) of infection while the lowest prevalence (10.86%) of infection was seen in the group of below 15 years of age. A relative study was also performed on caries patients according to their economic status and gender where Higher Middle Economic Class was in highest percentage (35.32%) of infection. Different species of bacteria showed various sensitivity patterns to several kinds of antibiotics. Regular use of tooth paste containing triclosan and fluoride may be beneficial for prevention of dental caries instead of using antibiotics presented in this study.

Asian J. Med. Biol. Res. June 2015, 1(2): 244-253


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Author Biography

Shuvho Chakra Borty, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh- 2202


Published
2015-11-23
How to Cite
Borty, S., Hafiz, K., Ali, M. M., Begum, K., Ahammed, T., Monir, M. S., & Islam, M. A. (2015). Isolation, identification and antibiogram profile of bacteria isolated from dental caries patients of Mymensingh district of Bangladesh. Asian Journal of Medical and Biological Research, 1(2), 244-253. https://doi.org/10.3329/ajmbr.v1i2.25618
Section
Articles