ELISA Based Anthrax Antibody Titer in Cattle Induced by Locally Prepared Anthrax Vaccine Originated from Sterne F-24 Strain in Bangladesh

Authors

  • Jayedul Hassan Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University (BAU), Mymensingh-2202
  • Md Bahanur Rahman Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University (BAU), Mymensingh-2202
  • Shah Md Ziqrul Haq Chowdhury Bangladesh Agricultural Research Council (BARC), Dhaka
  • Shushanto Kumar Rabidas Lal Teer Livestock Research and Development Farm, Bongao, Uthura, Valuka, Mymensingh
  • Md Shafiullah Parvej Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University (BAU), Mymensingh-2202
  • KHM Nazmul Hussain Nazir Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University (BAU), Mymensingh-2202

DOI:

https://doi.org/10.3329/mh.v4i1.23104

Keywords:

Cattle, ELISA, Antibody titer, Vaccine, Sterne F-24 strain, Bangladesh

Abstract

Vaccination is usually practiced to prevent and control anthrax in Bangladesh. For this purpose, vaccine prepared from Sterne F-24 strain ofBacillus anthracisby Livestock Research Institute (LRI), Mohakhali, Dhakahas long been used in this country. However, in some cases anthrax occurred in vaccinated animals in Bangladesh. A total of 100 cattle at LalTeer Livestock Research and Development Farm, LalTeerLivestock Limited, Bangladesh, aging between 3-6 years and weighing between 250-400 kg were randomly selected for vaccination purpose. Blood samples (n=100) were collected before the vaccination for collecting pre-vaccination serum, andthe animals were vaccinated (at 1 mL/animal; 1x107 spores/mL) with the anthrax vaccine produced by LRI. All blood samples from the vaccinated animals were collected on day 7, 28, 60, 90, 120, 150, 180, 240, 270, 300, 330, and 360 of post-vaccination, and serum samples were prepared. The antibody levels in the serum samples against anthrax were monitored using an Enzyme-Linked Immunosorbent Assay (ELISA). Over the course of 12 months, the antibody titers were found at the level higher than the reference value. Though there were reports on anthrax suspected cases in this farm, no such cases were reported during the study period. Thus, the vaccine appears to induce adequate antibody response against anthrax in Bangladesh.

Microbes and Health, January 2015. 4(1): 36-38

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Author Biography

Jayedul Hassan, Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University (BAU), Mymensingh-2202



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Published

2016-12-09

How to Cite

Hassan, J., Rahman, M. B., Chowdhury, S. M. Z. H., Rabidas, S. K., Parvej, M. S., & Nazir, K. N. H. (2016). ELISA Based Anthrax Antibody Titer in Cattle Induced by Locally Prepared Anthrax Vaccine Originated from Sterne F-24 Strain in Bangladesh. Microbes and Health, 4(1), 36–38. https://doi.org/10.3329/mh.v4i1.23104

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Section

Short Communications