In vitro Regeneration of Grass Pea (Lathyrus sativus L.)

  • P Saha Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902
  • M Afrin Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902
  • AKM Mohiuddin Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902
  • AM Shohael Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342
Keywords: Acclimatization, direct shoot regeneration, grass pea, organogenesis, root initiation

Abstract

In vitro regeneration protocol for grass pea (Lathyrus sativus L.) was optimized using different concentrations and combinations of growth regulators. Direct shoot regeneration obtained through shoot organogenesis from different explants of grass pea cultured on MS medium supplemented with Gamborg B5 vitamin containing 6-benzylaminopurine (BAP), Thidiazuron (TDZ) and ?-naphthalene acetic acid (NAA). Highest percentage of shoots were obtained at 4.0 mg/l of BAP on nodal explants. Stunted multiple shoots were developed from nodal explants while 1.5 mg/l TDZ was used. About 56% of direct shoots were also obtained, while the combination of BAP (4.0 mg/l) and NAA (0.5 mg/l) were used. Regenerated plantlets were rooted most effectively (40%) in rooting medium containing half strength of MS basal medium containing 1.0 mg/l NAA. Well rooted plantlets were further successfully acclimatized to ambient humidity level and grown in controlled environment until hardening.

Jahangirnagar University J. Biol. Sci. 4(2): 1-8, 2015 (December)

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Author Biography

P Saha, Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902


Published
2016-05-18
How to Cite
Saha, P., Afrin, M., Mohiuddin, A., & Shohael, A. (2016). In vitro Regeneration of Grass Pea (Lathyrus sativus L.). Jahangirnagar University Journal of Biological Sciences, 4(2), 1-8. https://doi.org/10.3329/jujbs.v4i2.27790
Section
Original Research Articles