Jahangirnagar University Journal of Biological Sciences https://www.banglajol.info/index.php/JUJBS <p>Official journal of the Faculty of Biological Sciences, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh</p> Faculty of Biological Sciences, Jahangirnagar University, Savar, Dhaka-1342 en-US Jahangirnagar University Journal of Biological Sciences 2306-0263 <p>(c) Jahangirnagar University Journal of Biological Sciences.  </p><p>Articles in the Jahangirnagar University Journal of Biological Sciences are Open Access articles published under the Creative Commons CC BY License (<a href="https://creativecommons.org/licenses/by/4.0/">https://creativecommons.org/licenses/by/4.0/</a>). This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.</p> Clonal propagation of Dracaena fragrans cv. Victoria through tissue culture technology https://www.banglajol.info/index.php/JUJBS/article/view/49833 <p>Micropropagation of <em>Dracaena fragrans </em>cv.Victoria was conducted using the young, tender and disease-free leaves and nodal segments as explants collected from the local market of Savar, Dhaka. Surface sterilization of the explants pretreated with a liquid detergent and then 0.2% HgCl2 for 4-5 minutes produces maximum contamination free explants without any toxicity. After surface sterilization, different explants were inoculated on gelrite gelled MS medium supplemented with different concentrations of 2,4-D for callus induction and with different concentrations and combinations of BAP and NAA for direct shoot induction. Nodal explants showed high callus induction potentiality (80%) on MS medium supplemented with 1.5 mg/l 2,4-D. The highest frequency of direct shoot induction from nodal segment was 80% and the number of shoots per nodal segment was(5.28±1.17) when they were cultured on MS medium supplemented with 3.0 mg/l BAP and 0.3 mg/l NAA. The highest shoot multiplication (83.33%) with maximum number of shoot per unit callus (5.62±1.24) and maximum shoot length (3.27±0.82 cm) was observed when the nodal calli were transferred in gelrite gelled MS medium in combination with 4.5 mg/l BAP and 0.5 mg/l NAA. Additionally, the incorporation of 4% sucrose and 10% coconut water with the above mentioned medium showed the satisfactory shoot growth and development with an average 7.84±1.30 shoots per unit of callus which was 4.21±0.78 cm in length. Moreover, addition of 3.0 mg/l GA3 with the above mention medium showed highest rate of shoot elongation (5.83±2.31cm). For root induction, <em>in vitro </em>raised shoots were transferred onto half-strength of MS liquid medium augmented with different concentrations and combinations of auxins (IBA and NAA). Maximum rooting (75%) were observed in halfstrength MS liquid medium supplemented with 0.5 mg/l IBA. After appropriate rooting the plantlets were successfully acclimatized (85% survival) when they were cultured in polybag containing (1:1:1) garden soil, sand and compost mixture before transferred to soil. Regenerated plants were morphologically identical with mother plants and showed their uniform growth in field condition.</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(2): 1-11, 2019 (December)</p> Ahshan Jazib Mohd Talim Hossain Raihan I Raju ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2020-10-19 2020-10-19 8 2 1 11 10.3329/jujbs.v8i2.49833 Checklist of the pteridophytes of Narsingdi District, Bangladesh https://www.banglajol.info/index.php/JUJBS/article/view/49834 <p>The occurrence of a total of 31 species of pteridophytes under 20 genera and 13 families in Narsingdi district were confirmed. Pteridaceae with 11 species was found as the largest family, which was followed by Salviniaceae and Polypodiaceae consisted of four species each and Thelypteridaceae with three species. Each of the rest nine families was represented by only one species. <em>Pteris </em>with six species was found as the largest genus, which was followed by <em>Salvinia </em>with three species, and <em>Adiantum</em>, <em>Ceratopteris</em>, <em>Pyrrosia </em>and <em>Cyclosorus </em>with two species each. Each of the rest 14 genera was represented by only one species. Among these species, 21 were found as terrestrial, two as both terrestrial and semiaquatic, four as aquatic and other four as epiphytic. The occurrence of <em>Tectaria chattagrammica </em>Ching, listed as DD (Data Deficient) in Red Data Book of Bangladesh, was common with natural regeneration in this district.</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(2): 13-24, 2019 (December)</p> Robayda Khanam Saleh Ahammad Khan Mohammod Abdur Rahim ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2020-10-19 2020-10-19 8 2 13 24 10.3329/jujbs.v8i2.49834 Effects of citric acid and gamma radiation on the shelf life of Labeo bata (Hamilton, 1822) https://www.banglajol.info/index.php/JUJBS/article/view/49838 <p>The effects of citric acid (2%) and gamma radiation (1.0 and 1.5 kGy) on the quality and shelf life extension of Bata, <em>Labeo bata </em>(Hamilton, 1822) stored at refrigerated temperature (4°C) for 28 days were examined. Quality assessment were made by sensory (organoleptic scores), chemical (TV) and microbiological (TBC and TCC) evaluations. Organoleptic evaluation showed that irradiated samples were more acceptable than control and citric acid treated samples which also remained acceptable upto 28 days. TV of fish muscles increased with the increase of storage periods in all the samples. The rate of increase was lower in citric acid treated samples and the lowest was observed in samples with 1.5 kGy irradiation. Irradiated samples showed the best results in microbiological assessment. Gamma radiation in combination with low temperature (4°C) could increase the shelf life, and this technique may be applied for other fish species preservation.</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(2): 25-33, 2019 (December)</p> - Kamrunnahar Md Enamul Haque Arzina Hossain Mahfuza Islam Md Kamruzzaman Munshi Khandoker Asaduzzaman Roksana Huque ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2020-10-19 2020-10-19 8 2 25 33 10.3329/jujbs.v8i2.49838 Arthropod ectoparasites of cattle and goats from three upazilas of Comilla district, Bangladesh https://www.banglajol.info/index.php/JUJBS/article/view/49835 <p>The prevalence of ectoparasitic arthropods of cattle and goats in Daudkandi, Homna and Titas upazilas of Comilla district were investigated. During this study (December 2013 to November 2014) 299 cattle and 196 goats were examined; and among them 180 and 117 were found to be infested respectively. Seventeen species of ectoparasites (both larvae and adult) were identified and they are <em>Haematopinus quadripertusus</em>, <em>Haematopinus eurysternus</em>, <em>Linognathus vituli, Damalinia bovis, Stomoxys calcitrans, Musca domistica, Tabanus striatus, Diachlorus </em>sp., <em>Armigeres subalbatus</em>, <em>Ctenocephalides felis</em>, <em>Boophilus microplus</em>, <em>Haemaphysalis bispinosa</em>, <em>Haemaphysalis kinneari</em>, <em>Hyalomma anatolicum</em>, <em>Rhipicephalus sanguineus</em>, <em>Dermacentor </em>sp. and <em>Ixodes </em>sp. The highest prevalence of ectoparasitic infestation (69.94%) were found in Titas and the lowest (53.34%) in Homna upazila. The intensity of ectoparasitic infestation was highest in Titas (12.20) and lowest in Homna (5.21) upazila. Cattle in Titas upazila showed highest prevalence (67.53%) and intensity (8.04) and Homna upazila showed lowest prevalence (48.63%) and intensity (5.55). Goats in Titas upazila showed highest prevalence (76.09%) and intensity (8.38), and Daudkandi upazila showed lowest prevalence (49.37%) and goats in Homna showed lowest intensity (4.80). This study quantifies the level of ectoparasitic infestation in cattle and goats which demands immediate control program and needs more intensive epidemiological study for detail identification of the constraints of animal health and production.</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(2): 35-45, 2019 (December)</p> I Mannan T Akter S Ahmad ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2020-10-19 2020-10-19 8 2 35 45 10.3329/jujbs.v8i2.49835 Reduction of Arsenate in a new isolate of Bacillus megaterium https://www.banglajol.info/index.php/JUJBS/article/view/49839 <p>In Bangladesh, the ground water of almost all the 64 districts are contaminated with arsenic and in some regions the arsenic concentration is above the World Health Organization’s guideline value. Bioremediation is in demand for its removal from water especially in rural areas. In this study, four soil samples were collected from arsenic contaminated areas of Chandpur, Bangladesh. In total 58 bacterial strains resistant to arsenate were isolated. Among them I-34 has the highest arsenate reducing capability. This bacteria showed resistance to high concentration of arsenite (100 mM) and arsenate (300 mM). Its arsenate reducing enzyme is extracellular and showed highest activity at 60 °C. More interestingly, it showed auto-induction metabolism to produce arsenate reducing enzyme. Molecular characterization by 16S rDNA gene sequence analysis revealed that the bacterium is 100% identical to <em>Bacillus megaterium</em>. I-34 appears to be novel arsenic metabolizing bacteria within this genus. The bacterial isolate can be exploited for the study of possible bioremediation of arsenic containing water and have a potential impact to reduce the arsenate into arsenite form.</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(2): 47-59, 2019 (December)</p> Md Zahidul Islam Mst Nusrat Jahan Arbi Ripa Moni Umme Salma Zohora Farzana Zafor Nishat Roksana Khanam Mohammad Shahedur Rahman ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2020-10-19 2020-10-19 8 2 47 59 10.3329/jujbs.v8i2.49839 Bioactivity evaluation of commercial root samples of Hemidesmus indicus (L.) R.Br. https://www.banglajol.info/index.php/JUJBS/article/view/49836 <p>Four commercial root samples (CS1, CS2, CS3 and CS4) of <em>Hemidesmus indicus </em>alongside an authentic sample (HI) were evaluated following antibacterial potential, cytototoxicity and DPPH free radical scavenging activity assay. The inhibition zones produced by commercial samples ranged between 9.5 to 13.5 mm against the tested bacteria in antibacterial sensitivity test. HI produced highest inhibition zone recorded as 20.63 mm against <em>Staphylococcus aureus </em>followed by 17.4 mm against <em>Bacillus subtilis </em>at 150 μg/ml concentration<strong>. </strong>HI also showed highest cytotoxicity with LC50 value of 1.726 mg/ml, 4.754 mg/ml and 13.247 mg/ml after 6 hours, 12 hours and 24 hours respectively in brine shrimp lethality assay. Sample CS1 and CS2 showed less cytotoxicity compared to CS3 and CS4. Depending on the concentration of extracts, the DPPH free radical scavenging activities of commercial samples were in close proximity with HI. The total phenolic content in HI was 54.52 mg/ml while in four commercial sample, it ranged between 52.28 to 75.37 mg/ml gallic acid equivalent (GAE) per 100 mg extract.</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(2): 61-69, 2019 (December)</p> Fatima Ferdouse Sanzida Mubassara Fakhruddin Ali Ahmed ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2020-10-19 2020-10-19 8 2 61 69 10.3329/jujbs.v8i2.49836 Tree species diversity of the Kaptai National Park in Rangamati district, Bangladesh https://www.banglajol.info/index.php/JUJBS/article/view/49837 <p>The purpose of this study was to estimate the tree biodiversity in the Kaptai National Park. The total area of the Kaptai National Park is about 4,564 ha (11,273.08 acres) under the jurisdiction of Rangamati South Forest Division, Rangamati Hill Tracts district. The study was conducted through extensive survey only on the tree species composition in the Kaptai National Park. During the investigation, a total of 65 tree species belonging to 29 families were recorded from the park. Among the plant families, Mimosaceae possessed the highest number of species (7) followed by Meliaceae (6), Ancardiaceae (5), Moraceae (4), Verbenaceae (4), Combretaceae (4), Myrtaceae (4), Dipterocarpaceae (3), Fabaceae (3), Rubiaceae (2), Bombacaceae (2), Caesalpiniaceae (2), Dilleniaceae (2) and Bignoniaceae (2) respectively. There were 15 families which contained single species. Tree species belonging to the family Mimosaceae was dominated in respect to number of species and their population as well. The findings of the present investigation will contribute in the regeneration of tree species in this degraded forest ecosystem and in the protection, conservation and sustainable management of the Kaptai National Park.</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(2): 71-79, 2019 (December)</p> Md Mukhlesur Rahman M Mahfuzur Rahman Md Kamrul Huda ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2020-10-19 2020-10-19 8 2 71 79 10.3329/jujbs.v8i2.49837 Prevalence of birth abnormalities and congenital malformations in progenies from parental consanguinity in Rajshahi City Corporation area, Bangladesh https://www.banglajol.info/index.php/JUJBS/article/view/49840 <p>In a series of two separate studies during January through August 2018, various birth abnormalities (BA) from 178 clinical cases and congenital malformations (CMF) from 70 consanguineous marriages (CM) and 100 non-consanguineous marriages (NCM) within the Rajshahi City Corporation (RCC) area have been recorded, analyzed and interpreted. Results demonstrate that frequencies of miscarriages (5.69 <em>vs. </em>3.00), stillbirths (3.85 <em>vs. </em>1.00), postnatal deaths (8.97 <em>vs. </em>1.00), preterm deliveries (29.49 <em>vs. </em>38.00) and breech births (6.41 <em>vs. </em>4.00) were significantly greater in CM compared to the NCM counterparts. In addition, both gestational ages and live birth weights of the children from CM were significantly lesser than those recorded from the NCM couples. Of 205 children derived from 70 CM couples 67 were suffering from various CMF, the remaining 138 children were normal. Compared to this, only 2 out of 267 children were affected from 100 NCM couples. Mental retardation (MR) represented the highest CMF (n= 37), followed by cerebral palsy (CP; n= 16), crossed-eyes (CE; n=4), blindness (BL) and microcephaly (MC; n= 3 each), deaf-mute (DM; n= 2), and Down’s syndrome (DS) and syndactyly (SD; n= 1 each). In contrast to the CM cases, the NCM couples had only two affected children, one crossed-eyed girl and the other polydactylous boy. The overall frequency of CMF in CM (32.68) was much higher than that in NCM cases (0.75). In relation to parental consanguinity, the importance of genetic counselling and pre-implantation screening relevant to the present findings has been emphasized in this report.</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(2): 81-91, 2019 (December)</p> M Saiful Islam Sharmin Mustari ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2020-10-19 2020-10-19 8 2 81 91 10.3329/jujbs.v8i2.49840