Jahangirnagar University Journal of Biological Sciences 2020-04-18T16:26:04+00:00 Professor Abdul Jabber Howlader, Ph.D. Open Journal Systems <p>Official journal of the Faculty of Biological Sciences, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh</p> Effect of Ashwagandharista (Withania somnifera) on the kidney functions of male and female rats 2020-04-18T16:25:53+00:00 Tasmina Rahman Md Rakib Hasan MSK Choudhuri <p>The key objective of this present study was to analyze the effect(s) of Ashwagandharishta on the kidney functions of both male and female Albino rats. Chronic toxicity tests were also done. Following treatments the rats were observed for 51 days to know the effects of Ashwagandharishta on kidney functions considering 3 parameters such as serum urea, creatinine and uric acid. Our results failed to exhibit a significant increase in serum urea level at low dose (P&lt;0.01), medium dose (P&lt;0.05) and at high dose (P&lt;0.001) in male rat groups; but with female rat groups our results showed significant increase in serum urea level at three dose levels. Regarding serum creatinine level male rats and female rats showed a trend of increase in level at different dose but effects were insignificant except medium dose in male rats (P&lt;0.05). Regarding serum uric acid level our results failed to show a significant increase irrespective of dose.</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(1): 1-7, 2019 (June)</p> 2019-08-03T18:01:13+00:00 ##submission.copyrightStatement## Characterization of rhizospheric and non rhizospheric bacteria from arsenic contaminated soil 2020-04-18T16:25:43+00:00 Umma Mayda Nazifa Tasnim Rasheda Yasmin Shilpi <p>The rhizosphere soil has a large and various number of microorganisms especially the bacteria. This experiment was conducted at the department of Botany, Jahangirnagar University, Savar, to investigate the rhizospheric and non-rhizospheric bacteria from arsenic contaminated soil. <em>Pteris vittata </em>was treated with different concentrations of arsenic ranges from 5000 to 10000 ppm in the pot. The experimental result indicated that the negative correlation between arsenic concentration and rhizospheric soil bacteria. Highest number of bacteria (8.6×10<sup>8</sup> cfu/g) were found in rhizospheric soil (control), while lowest numbers of bacteria (4.0×10<sup>7</sup> cfu/g) were found in the non-rhizospheric soil with 10000 ppm arsenic. Thirty bacteria were isolated from rhizospheric and non-rhizospheric soil samples. Out of thirty samples <em>Bacillus </em>and <em>Pseudomonas </em>were selected on the basis of morphological and biochemical nature. The present study concluded that the arsenic has an adverse effect on the growth of rhizospheric soil bacteria.</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(1): 9-15, 2019 (June)</p> 2019-08-03T18:01:21+00:00 ##submission.copyrightStatement## Food and feeding behaviour of Chestnut-tailed Starling, Sturnia malabarica at Jahangirnagar University Campus, Bangladesh 2020-04-18T16:26:04+00:00 Md Touhidur Rahman Shamia Farhana Shoma Mohammed Mostafa Feeroz Md Kamrul Hasan <p>Food and feeding behaviour of Chestnut-tailed Starling, <em>Sturnia malabarica </em>were studied at Jahangirnagar University Campus, Bangladesh, from August 2016 to March 2017. A total of 414 observations were made on the feeding maneuver and it was noted that they were omnivorous consuming 67.15% animal diet compared to 20.53%plant diet.They predominantly consumedinsect larvae(39%) followed by beetles (16%), nectar (14%), food wastes (12%), fruits (7%), dragonflies (7%), damselflies (3%), and worms (2%).Among the five types of feeding modes recorded,hang-upmode (37.92%) was major feeding technique in Chestnuttailed Starling while pecking mode (6.76%)was least used. Rain tree (<em>Samanea saman</em>) (33.76%) followed by White siris (<em>Albizia procera</em>) (30.55%) was recorded as the most utilized foraging plant while mostly preferred perching height by Chestnut-tailed Starling was 6-9m (44.9%) followed by 3-6m (31.6%).</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(1): 17-23, 2019 (June)</p> 2019-08-03T00:00:00+00:00 ##submission.copyrightStatement## Proximate composition of two puffer fish species, Leiodon cutcutia and Dichotomyctere fluviatilis of Bangladesh 2020-04-18T16:25:33+00:00 Ishtiak Ahmed Chowdhury Md Rakibul Hasan Suraiya Parveen Shuvra Kanti Dey Md Baki Billah <p>Protein, lipid, ash and moisture content in the body muscles of two commonly available puffer fish species in Bangladesh (<em>Leiodon cutcutia </em>and <em>Dichtomyctere fluviatilis</em>) have been analysed from January 2018 to June, 2018. The puffer fish species were collected from different habitats i.e., <em>Leiodon cutcutia </em>from freshwater and <em>Dichtomyctere fluviatilis </em>from estuarine water. The percentage of the proximate composition varied in different months in both the species. In <em>Leiodon cutcutia</em>, moisture content fluctuated from 79.32% to 87.61% with an average of 83.75%; protein content from 6.35% to 13.31% with an average of 9.49%; lipid content from 1.57% to 2.32% with an average of 1.92% and ash content from 2.30% to 3.27% with an average of 2.84%. In <em>Dichotomyctere fluviatilis</em>, moisture content fluctuated from 73.77% to 84.18% with an average of 77.49%; protein content from 10.03% to 19.77% with an average of 16.80%; lipid content from 0.92% to 1.71% with an average of 1.32% and ash content from 2.77% to 3.34% with an average of 3.05%. The comparative evaluation of the nutritional value of the studied fish species revealed that the puffer fish from estuarine environment might contain higher amount of nutrients. Thus, proper utilization of this species towards sustainable management, nutritional composition and biosecurity issues will lead to achieve sustainable blue economy.</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(1): 25-33, 2019 (June)</p> 2019-08-03T18:02:01+00:00 ##submission.copyrightStatement## Neuropharmacological and gastrointestinal evaluation of coloring agent metanil yellow used in food and beverages 2020-04-18T16:25:23+00:00 Md Arfanur Rahman Arpon Krishna Bala Md Ataur Rahman Md Kamrul Hasan Runa Masuma <p>Metanil yellow as a coloring agent is widely used in food industry to make food more appealing, appetizing and informative. Regulatory organization like FDA maintains strict rules but in our country coloring agents are at randomly used. This study is designed to evaluate side effects of metanil yellow on central nervous system and gastrointestinal tract in mice. Open field, hole board and hole cross tests were done to evaluate the effects of central nervous system; while castor oil and magnesium sulfate induced diarrheal tests were done to observe the effects on gastrointestinal tract. The mice were administered a dose of 5 gm/kg body weight of metanil yellow considered as 1x dose (MTYx) and another dose of 20 gm/kg body weight of metanil yellow considered as 4x dose (MTY4x). In the study, the metanil yellow exhibited central nervous system stimulatory effects based on hole cross test (p=000***, p=.003**, p=000***, p=.001***), hole board test (p=.03*, p=.005**, p=.041*, p=0.018*), and open field test (p=0.004**, p=.002**, p=.002**, p=.011*, p=0.008*). On the other hand gastrointestinal test results failed to show a significant effect (p=.04*).</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(1): 35-44, 2019 (June)</p> 2019-08-03T18:02:11+00:00 ##submission.copyrightStatement## Establishment of a functional and sustainable bio-system engineering process for the saccharification of starch with industrial focus 2020-04-18T16:25:12+00:00 Shatabdy Saha Md Zahidul Islam Umme Salma Zohora Mohammed Salahuddin Sabbir Janee Mohammad Shahedur Rahman <p>Enzyme immobilization provides an excellent opportunity to use the enzymes for several times with greater productivity. The main aim of the present study is the establishment of a functional and sustainable bio-system engineering process based on immobilized α-amylase enzyme for the saccharification of starch with industrial focus as well as cost effective sustainable bioprocess system by using indigenous industrial waste materials as carrier agents. In this study, α-amylase was immobilized in different types of support matrices like alginate, sawdust, sugarcane bagasse and the activity of immobilized enzymes were analyzed for the breakdown of starch. The experimental results showed that the productivity of immobilized enzyme was around 2.3 times higher than the free enzyme. Conjugates of bagasse-alginate showed the highest result. A fixed batch immobilized enzyme bioreactor had been developed which could be used for the production of various valuable products in the industrial scale.</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(1): 45-55, 2019 (June)</p> 2019-08-03T18:02:21+00:00 ##submission.copyrightStatement## Study of an arsenic metabolizing bacteria from arsenic contaminated soil of Chandpur district, Bangladesh 2020-04-18T16:25:02+00:00 Roksana Khanam Ripa Moni Md Zahidul Islam Md Morsaline Billah Umme Salma Zohora Farah Sabrin Mohammad Shahedur Rahman <p>Arsenic is a toxic metal found as inorganic oxyanion arsenate As(V) and arsenite As (III) species. The disposal of toxic heavy metals such as arsenic poses high risk to environment. The present study was undertaken to isolate arsenic-metabolizing bacteria from arsenic contaminated soil of Chandpur district, which is one of the most arsenic contaminated area in Bangladesh and later these bacteria were screened for their ability to metabolize arsenate. Out of ninety eight isolates, ten were found to be capable of metabolizing arsenic in Yeast Extract Mannitol (YEM) medium containing 2 mM arsenate at 37ºC. One of the bacterial isolates designated as I-25 was found to produce an extracellular enzyme which can reduce As(V) into As(III) and able to grow in presence of up to 500 mM arsenate. Subsequent molecular identification of this enzyme producing bacterial isolate using 16s rRNA sequence analysis was correlated with previously identified isolate as <em>Bacillus aryabhatti. </em>Further characterization of the enzyme showed that optimum pH of the extracellular enzyme by the bacterial species was 7 and optimum temperature for the enzyme activity was 60ºC. The bacterial isolates can be exploited for the study of possible bioremediation of arsenic contamination.</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(1): 57-65, 2019 (June)</p> 2019-08-03T18:02:29+00:00 ##submission.copyrightStatement## Molecular identification and phylogenetic relationships of seven Satyrinae butterflies in Bangladesh using Cytochrome c oxidase subunit I gene 2020-04-18T16:24:53+00:00 Ananna Ghosh Muhammad Sohel Abedin Abdul Jabber Howlader Md Monwar Hossain <p>The Satyrinae is a subfamily of Nymphalid butterfly, which is morphologically and ecologically the most diverse group, occurring in all habitats. In the present study, Cytochrome <em>c </em>oxidase subunit I (<em>COI</em>) gene of seven species of Satyrinae was sequenced, aligned, and used to construct phylogenetic trees. The molecular identification of these Satyrinae species was confirmed by comparing the related sequences in the National Center for Biotechnology Information (NCBI) GenBank. The base compositions of the <em>COI </em>sequences were 39.07% T, 16.44% C, 29.83% A, and 14.64% G, revealing a strong AT bias (68.9%). The sequence distance among Satyrinae species ranged from 0.09% to 0.18%. Phylogenetic trees were constructed by the neighbor-joining (NJ) and maximum likelihood (ML) methods, using <em>Orthetrum sabina </em>as an outgroup. Both trees had almost identical topologies. The sampled species in Satyrinae exhibited the following relationships: <em>Melanitis leda </em>+ [(<em>Mycalesis mineus</em>+(<em>Mycalesis gotama</em>+<em>Mycalesis anaxias</em>)) + (<em>Ypthima baldus </em>+ (<em>Lethe chandica</em>+<em>Elymnias hypermnestra</em>))], suggesting that <em>M. leda </em>might be distantly related with the rest of the Satyrinae species. This clustering result is almost identical to current traditional classification. This study confirms that the <em>COI </em>based DNA barcoding is an efficient method for the identification of butterflies including Satyrinae species and, as such, may further contribute effectively to biodiversity and evolutionary research.</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(1): 67-74, 2019 (June)</p> 2019-08-03T18:02:38+00:00 ##submission.copyrightStatement## Prevalence and abundance of insect pests in stored pulses collected from two local markets of Dhaka city 2020-04-18T16:24:43+00:00 Tangin Akter Shanjida Sultana Mehjabin Rahman Md Reaz Mahmud Shefali Begum <p>Prevalence and abundance of stored grain insect pests in four varieties of pulses i.e. <em>Lens culinaris</em>, <em>Pisum staivum</em>, <em>Cicer arietinum </em>collected from two markets of Dhaka city were recorded. Nine species of insect pests, <em>viz. Sitophilus oryzae, Tribolium castaneum, Callosobruchus chinensis, C. maculatus, Rhizopertha dominica, Oryzaephilus surinamensis, Liposcelis entomophila, Sitotroga cerealella, </em>and <em>Formica </em>sp., were recorded throughout the study. The percentage of weight-loss was higher (7.54) in those pulses which were highly infested by pests. Highest pest population was observed during May to June (753). However, <em>Sitotroga cerealella </em>were found only in November to December of the study period. The maximum species richness (7) was observed in Green gram and Chickpeas samples, but the minimum species evenness was observed in Green gram (0.6969) and maximum in Red lentil (0.9886).</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(1): 75-82, 2019 (June)</p> 2019-08-03T18:02:45+00:00 ##submission.copyrightStatement## Effect of temperature and relative humidity on the population dynamics of brinjal and tomato infesting whitefly, Bemisia tabaci 2020-04-18T16:24:33+00:00 MMH Khan <p>Abstract not available</p> <p>Jahangirnagar University J. Biol. Sci. <strong>8</strong>(1): 83-86, 2019 (June)</p> 2019-08-03T18:02:54+00:00 ##submission.copyrightStatement##