Isolation and Identification of Mycobacterium from Extrapulmonary Specimen at NTRL, NIDCH

Authors

  • SM Mostofa Kamal Assistant Professor, Pathology and Microbiology and Coordinator, NTRL, NIDCH, NTP, DGHS, Mohakhali, Dhaka
  • HAM Nazmul Ahasan Assistant professor, Department of Medicine, Dhaka Medical College Hospital, Dhaka
  • Sharmin Ahmed Post-graduate trainee, Department of Medicine, Dhaka Medical College, Hospital
  • KFM Ayaz MD (Internal Medicine), Dhaka Medical College, Hospital
  • Md Shahriar Mahbub Post-graduate trainee, Department of Medicine, Dhaka Medical College, Hospital
  • Mohammad Ashik Imran Khan Post-graduate trainee, Department of Medicine, Dhaka Medical College, Hospital
  • Ratan Das Gupta Assistant Professor, Department of Medicine, Dhaka Medical College, Hospital
  • Md Billal Alam Associate Professor, Department of Medicine, Dhaka Medical College
  • Md Titu Miah Assistant Professor, Department of Medicine, Dhaka Medical College, Hospital

DOI:

https://doi.org/10.3329/jom.v11i2.5454

Keywords:

Pulmonary tuberculosis, extrapulmonary, Microbial Sensitivity Test, Laboratory Technics and Procedures, Bangladesh

Abstract

Objective of the study was to see the frequency of isolation of Mycobacterium among different extrapulmonary
specimens. The study was carried out at NTRL (National Tuberculosis reference laboratory), NIDCH Bangladesh
during January 2008-June 2009.This study was carried out retrospectively by analyzing NTRL laboratory data. A
total of 514 extra-pulmonary specimens from different treatment centre of Dhaka was analyzed. Clinical specimens,
such as lymph node aspirate, pleural fluid, urine, stool, gastric lavage, pus, ascitic fluid, cerebrospinal fluids, etc was
collected. Lowenstein-Jensen media was used for culture and antimicrobial susceptibility testing. Mycobacteria were
isolated from 113 extra-pulmonary specimens. Male and female ratio was almost equal among positive cases. The
commonest source of isolation was lymph nodes(frequency 55.8%) and lymph node aspirate( frequency 68.4%)
pleural fluid (frequency10.6%).Anti-microbial susceptibility of the isolates to the four first line anti-tuberculosis drugs,
rifampicin, isoniazid, streptomycin and ethambutol was tested, susceptibility rate was 100%.The results suggest that,,
emphasis should be placed on laboratory diagnosis and treatment of extra-pulmonary tuberculosis.

Keywords: Pulmonary tuberculosis; extrapulmonary; Microbial Sensitivity Test; Laboratory Technics and Procedures;
Bangladesh

DOI: 10.3329/jom.v11i2.5454

J MEDICINE 2010; 11 : 128-130

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How to Cite

Kamal, S. M., Ahasan, H. N., Ahmed, S., Ayaz, K., Mahbub, M. S., Khan, M. A. I., Gupta, R. D., Alam, M. B., & Miah, M. T. (2010). Isolation and Identification of Mycobacterium from Extrapulmonary Specimen at NTRL, NIDCH. Journal of Medicine, 11(2), 128–130. https://doi.org/10.3329/jom.v11i2.5454

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Original Articles