The Effect of Perchloroethylene (PCE) Exposure Toward Frequency of Micronucleus Formation in Buccal Mucosal Ephitelia of Dry Cleaning Employees
Keywords:Perchloroethylene, micronucleus, dry cleaning
Background: Perchloroethylene is one of the main ingredients used in the dry cleaning industry. Continuous inhalation of Perchloroethylene particles can cause genetic damage that can be marked by the appearance of micronucleus. Micronucleus formation of excessive buccal mucosal is one of the early signs of malignancy in the oral cavity.
Objective: To determine the effect of Perchloroethylene exposure toward frequency of micronucleus formation in buccal mucosal epithelial of dry cleaning employees in Banyumas Regency.
Materials & methods: This study uses cross-sectional design with primary data that is the preparation of buccal mucosal smear from 22 dry cleaning employees as exposed group and 22 subjects are not exposed to Perchloroethylene as control group. The buccal epithelial cells are collected using a spatula and then smeared on an object glass and colored using Giemsa. Micronuclear frequencies are calculated per 1000 cells using a light microscope.
Results: The difference of micronucleus frequency between two groups (p = 0.000) is obtained. The duration of work has correlation with medium correlation strength to the forming frequency of micronucleus formation, whereas age has strong correlation to the forming frequency of micronucleus formation. The correlation between age and work duration with the forming frequency of micronucleus formation is significant (p = 0.000 and p = 0.07)
Conclusions: There is influence of Perchloroethylene exposure toward frequency of micronucleus formation. The duration of work and age are significantly correlated with the forming frequency of micronucleus formation.
J MEDICINE JUL 2019; 20 (2) : 59-62
How to Cite
LicenseAuthors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).