<i>In Vitro</i> Micropropagation of Orchid (<i>Vanda tessellata</i> L.) from Shoot Tip Explant

  • MS Rahman Department of Genetic Engineering & Biotechnology, University of Rajshahi
  • MF Hasan Department of Genetic Engineering & Biotechnology, University of Rajshahi
  • R Das Department of Genetic Engineering & Biotechnology, University of Rajshahi`
  • MS Hossain Department of Genetic Engineering & Biotechnology, University of Rajshahi
  • M Rahman Department of Genetic Engineering & Biotechnology, University of Rajshahi
Keywords: Shoot tip, micropropagation, orchid

Abstract

Context: Orchid produces a huge number of minute seeds but the seeds can not germinate easily in nature due to the lack of endosperm in the seeds is an incompatibility barrier that limits its propagation in nature.

Objectives: To develop in vitro culture techniques for quick propagation of Vanda tessellate, a commercially important orchid species.

Materials and Methods: Shoot tips were used as experimental materials. The explants were surface sterilized and the shoot tips were excised. The isolated shoot tips were cultured in MS medium supplemented with different concentration and combinations of auxin and cytokinin.

Results: The combination of 1.5 mgl-1 NAA and 1.0 mgl-1 BAP was proved to be the best medium formulation for multiple shoot formation as well as maximum shoot elongation. The single shoots were isolated from the multiple shoots and subcultured in MS medium having NAA and IBA individually and in combinations for root induction. Maximum root induction was obtained in MS agarified medium having 0.5 mgl-1NAA and 1.0 mgl-1IBA. The well rooted plantlets were hardened successfully in the potting mixture containing coconut husk, perlite, charcoal, brick pieces in the ratio of 2:1:1:1 and eventually established under natural condition.

Conclusion: An efficient regeneration protocol for micropropagation in V. tessellata through shoot tip culture has been established.

Key words: Shoot tip; micropropagation; orchid.

DOI: 10.3329/jbs.v17i0.7122

J. bio-sci. 17: 139-144, 2009

Downloads

Download data is not yet available.
Abstract
2884
PDF
1194
How to Cite
Rahman, M., Hasan, M., Das, R., Hossain, M., & Rahman, M. (1). <i>In Vitro</i> Micropropagation of Orchid (<i>Vanda tessellata</i&gt; L.) from Shoot Tip Explant. Journal of Bio-Science, 17, 139-144. https://doi.org/10.3329/jbs.v17i0.7122
Section
Articles