Antibacterial Activity and Cytotoxicity of Three Lectins Purified from Drumstick (<i>Moringa oleifera</i> Lam.) Leaves

  • Shahanaz Khatun Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi
  • MMH Khan Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi
  • M Ashraduzzaman Department of Chemistry, Rajshahi University of Engineering and Technology, Rajshahi
  • Farzana Pervin Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi
  • Luthfunnessa Bari Department of Food Technology and Nutritional Science, Mawlana Bhashani Science and Technology University, Tangail
  • N Absar Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi
Keywords: Drumstick, Moringa oleifera, lectins, antibacterial activity, brine shrimp, bioassay

Abstract

Context: Plant materials contain glycoproteins (phytolectins) that are toxic in nature may play a key role in the control of various normal and pathological processes in living organisms and have diverse biochemical and diagnostic applications.

Objectives: Screening of three lectins SLL-1, SLL-2 and SLL-3 purified from Drumstick (Moringa oleifera Lam.) leaves for their antibacterial activities and brine shrimp lethality bioassay.

Materials and Methods: Three bioactive lectins were purified from Drumstick leaves by conventional chromatographic methods. The lectins were tested for their antibacterial activities against three pathogenic bacteria- Escherichia coli (gram-negative) Shigella dysenteriae (gram-negative) and Staphylococcus aureus (gram-positive) using the standard disc-diffusion method. Mortality of the brine shrimp naupli was assessed by hality bioassay.

Results: All the lectins showed antibacterial activity against E. coli, Sh. dysenteriae and St. aureus. They also showed cytotoxic effect in brine shrimp (Artemia salina L.) lethality bioassay. The LC50 values of SLL-1, SLL-2 and SLL-3 were found to be 15.8, 17.78 and 14.12 μg/ml respectively. The experimental results revealed that SLL-3 is more cytotoxic than other lectins. The lectin SLL-3 showed lowest activity whereas SLL-1 showed highest activity against the three bacteria.

Conclusion: Results suggest that the extracts from M. oleifera leaf can be a source of natural antimicrobials with potential applications in pharmaceutical industry to control coliform bacteria.

Key words:  Drumstick; Moringa oleifera; lectins; antibacterial activity; brine shrimp; bioassay

DOI: 10.3329/jbs.v17i0.7112

J. bio-sci. 17: 89-94, 2009

Downloads

Download data is not yet available.
Abstract
799
PDF
741
How to Cite
Khatun, S., Khan, M., Ashraduzzaman, M., Pervin, F., Bari, L., & Absar, N. (1). Antibacterial Activity and Cytotoxicity of Three Lectins Purified from Drumstick (<i>Moringa oleifera</i&gt; Lam.) Leaves. Journal of Bio-Science, 17, 89-94. https://doi.org/10.3329/jbs.v17i0.7112
Section
Articles