Effect of Carbon Sources on Cell Growth and Regeneration Ability in Three Cultivars of Banana
Keywords:Banana, Musa, callus, single cell, regeneration
AbstractContext: Carbon plays a vital role in plant cell growth and regeneration in artificial media but the source of carbon deserves scientific investigation to analysis their comparative performance.
Objectives: To analyze the comparative performance of different carbon sources (glucose, sucrose and sorbitol) in cell growth and regeneration efficiency of banana (Musa spp) cultivars.
Materials and Methods: Male flowers of banana cultivars cv. Sabri, Gine and Ranginsagar were used in this experiment. Male flowers were cut into small pieces and they were transferred in petri dishes containing Murashige and Skoog media supplemented with 2 mg/l 2,4-D + 1mg/l NAA + 1mg/l IAA + 1mg/l Biotin + 1mg/l glutamine and 3% (w/v) different sugars: sucrose, glucose, and sorbitol singly or in combinations autoclaved in 121ºC temperature for 20 min. The pH of the medium was adjusted to 5.8.
Results: Glucose showed the highest performance in callus induction and cell growth and 3% glucose proved as the optimal dose in media formulation for callus induction and cell growth. Sucrose and sorbitol behaves differently in embryo formation and they produced the highest and lowest number of embryos respectively in regeneration medium. In respect of overall performance the highest percentages of shoot and root formation was obtained in the media containing 3% sucrose.
Conclusion: Glucose proved to be the best carbon source in callus induction and cell growth media.
Key words: Banana; Musa; callus; single cell; regeneration
J. bio-sci. 17: 83-88, 2009