<i>In Vitro</i> Propagation of Pumpkin and Ash Gourd through Nodal Segments

  • ME Haque Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi 6205, Banglade
  • MAR Sarkar Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi 6205, Banglade
  • MA Mahmud Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi 6205, Banglade
  • D Rezwana Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi 6205, Banglade
  • B Sikdar Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi 6205, Banglade
Keywords: In vitro, MS medium, nodal segments, multiplication, rooting, pumpkin and ash gourd

Abstract

The present study was undertaken with a view to develop an efficient protocol for in vitro multiple shoot formation and subsequent root induction considering various cultural aspects using nodal segments of Cucurbita maxima and Benincasa hispida. The best hormone for shoot multiplication of pumpkin was BAP (2 mg/l), incase of ash-gourd BAP was 1.5 mg/l. For callus induction BAP+2,4-D was best combination for pumpkin and it was 2.0+0.1 mg/l when in ash gourd BAP+NAA was the best combination. IBA + ½MS medium were used for induction of shoot bud root. In both of pumpkin and ash gourd 1.5 mg/l IBA was found best for induction of roots.

Key words: In vitro, MS medium, nodal segments, multiplication, rooting, pumpkin and ash gourd.  

DOI:10.3329/jbs.v16i0.3743

J. bio-sci. 16: 67-71, 2008

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How to Cite
Haque, M., Sarkar, M., Mahmud, M., Rezwana, D., & Sikdar, B. (1). <i>In Vitro</i&gt; Propagation of Pumpkin and Ash Gourd through Nodal Segments. Journal of Bio-Science, 16, 67-71. https://doi.org/10.3329/jbs.v16i0.3743
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