Micropropogation of <i>Cucurbita maxima</i> Duch. through Shoot Tip Culture
Micropropagation of pumpkin (Cucurbita maxima Duch.) was achieved using shoot tip of in vitro grown seed derived plants of two cultivars namely, Bikrompuri and Baromasi of Bangladesh. The excised shoot tips were cultured on MS medium containing KIN, BA, NAA at various levels of concentration and combination for shoot induction and proliferation, and best response was found at 3.0 mg/l of BA. Shoots were rooted most effectively in ½ MS medium supplemented with 1.0 mg/l IBA. Bikrompuri was found more responsive than Baromasi for rapid clonal propagation.
Key words: Micropropagation, Direct organogenesis, Shoot tip, Pumpkin
J. bio-sci. 16: 59-65, 2008