In Vitro Plant Regeneration from Cultured Cotyledons of Cotton (Gossypium herbaceum L.)

Authors

  • AN Chowdhury Applied Botany Researeh Division, BCSIR Labratories, Rajshahi
  • MZ Rahman Applied Botany Researeh Division, BCSIR Labratories, Rajshahi
  • A Samad Applied Botany Researeh Division, BCSIR Labratories, Rajshahi
  • AKMS Alam Applied Botany Researeh Division, BCSIR Labratories, Rajshahi
  • S Khaleda Applied Botany Researeh Division, BCSIR Labratories, Rajshahi

Keywords:

In vitro, Callus, Cotyledons, Cytokinin, Plantlet, Acclimatization

Abstract

The effect of cytokinins on callus proliferation from cotyledons and plantlet development was studied in cotton. The frequency of callus induction was observed on MS medium enriched with a variety of cytokinins in different concentrations. With the increase of cytokinin concentration, the percentage of callus formation, percentage of shoot developing calli and number of shoots/calli were increased. Among the three different cytokinins studied, BA showed the highest performance. The highest percentage of callus (6.55%) and shoot developing calli (5.87%) was obtained on MS with 1.0 mg/l BA. Highest number of shoots (3.02) per calli was observed on MS media supplemented with 1.0 mg/l Kn. The rooting media composed of MS medium, 0.6% agar, sucrose and fortified with 2.0 mg/l NAA induced root development at the highest percentage (41.23%) with maximum number of roots (3.61) per cutting and length of root (3.62 cm) per culture. The plantlets were acclimatized in natural conditions.

Key words: In vitro; Callus; Cotyledons; Cytokinin; Plantlet; Acclimatization

DOI: http://dx.doi.org/10.3329/bjsir.v46i3.9043

BJSIR 2011; 46(3): 359-364

Abstract
267
PDF
322

Downloads

How to Cite

In Vitro Plant Regeneration from Cultured Cotyledons of Cotton (Gossypium herbaceum L.). (2011). Bangladesh Journal of Scientific and Industrial Research, 46(3), 359-364. https://doi.org/10.3329/bjsir.v46i3.9043

Issue

Vol. 46 No. 3 (2011)

Section

Articles

License

Bangladesh Council of Scientific and Industrial Research (BCSIR) holds the copyright to all contents published in Bangladesh Journal of Scientific and Industrial Research (BJSIR). A copyright transfer form should be signed by the author(s) and be returned to BJSIR.

The entire contents of the BJSIR are protected under Bangladesh Council of Scientific and Industrial Research (BCSIR) copyrights.

Creative Commons Licence
BJSIR is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC) Creative Commons Attribution-NonCommercial 4.0 International License which allows others remix, tweak, and build upon the articles non-commercially, and although their new works must also acknowledge and be non-commercial, they don’t have to license their derivative works on the same terms.

How to Cite

In Vitro Plant Regeneration from Cultured Cotyledons of Cotton (Gossypium herbaceum L.). (2011). Bangladesh Journal of Scientific and Industrial Research, 46(3), 359-364. https://doi.org/10.3329/bjsir.v46i3.9043