Comparison of results obtained by widal agglutination test & polymerase chain reaction among clinically suspected typhoid fever cases

  • Shafinaz Khan Assistant Professor, Department of Microbiology, Dr. Sirajul Islam Medical College and Hospital, Dhaka
  • Md Ruhul Amin Miah Professor, Department of Microbiology, Bangabandhu Shiekh Mujib Medical University, Dhaka
  • Shammin Haque Assistant Professor, Department of Pharmacology, Dr. Sirajul Islam Medical College and Hospital, Dhaka
  • Chowdhury Rafia Naheen MD resident (Thesis part), Department of Microbiology, Bangabandhu Shiekh Mujib Medical University, Dhaka
Keywords: Typhoid fever, nested PCR, widal test

Abstract

The diagnosis of typhoid fever currently depends on isolation of Salmonella Typhi from blood. The sensitivity of blood culture is very low due to prior antibiotic treatment which is a common practice in Bangladesh. The sensitivity of blood culture also decreases at later stage of the disease. Widal test is the most utilized test in Bangladesh next to blood culture because it is inexpensive, less invasive. But the result of the test is controversial due to false negative & false positive results in some cases.  In this study, a recently introduced polymerase chain reaction-based technique (which has 100% specificity for S. Typhi) was compared with widal test among 80 clinically suspected typhoid fever cases.  Among 80 cases, the respective figures of positivity for PCR & widal test were 70% & 43.75% respectively.  It can be concluded that PCR based technique is more sensitive & much superior to widal for diagnosis of typhoid fever.

DOI: http://dx.doi.org/10.3329/bjpp.v30i2.22683

Bangladesh J Physiol Pharmacol 2014; 30(2): 46-50

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Abstract
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Published
2015-03-25
How to Cite
Khan, S., Miah, M. R., Haque, S., & Naheen, C. (2015). Comparison of results obtained by widal agglutination test & polymerase chain reaction among clinically suspected typhoid fever cases. Bangladesh Journal of Physiology and Pharmacology, 30(2), 46-50. https://doi.org/10.3329/bjpp.v30i2.22683
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Original Articles