Lactucin induces potent anti-cancer effects in HL-60 human leukemia cancer cells by inducing apoptosis and sub-G1 cell cycle arrest

Authors

  • Fu-Hua Zhang Department of Hematology, Dongying People?s Hospital, Dongying City, Shandong 257091
  • Yu-Ling Yan Department of Hematology, Dongying People?s Hospital, Dongying City, Shandong 257091
  • Ying Wang Department of Hematology, Dongying People?s Hospital, Dongying City, Shandong 257091
  • Zhen Liu Department of Pharmacy, Dongying People?s Hospital, Dongying City, Shandong 257091

DOI:

https://doi.org/10.3329/bjp.v11i2.26729

Keywords:

Anti-cancer, Apoptosis, HL-60 human leukemia cancer cell, Lactucin, Sub-G1 cell cycle arrest

Abstract

The main purpose of the present study was to examine the antitumor and apoptotic effects of lactucin in HL-60 human leukemia cancer cells. MTT assay was used to examine the cytotoxic effects of lactucin while as phase contrast, fluorescence and transmission electron microscopy techniques were used to evaluate the apoptotic effects of lactucin in these cells. Flow cytometry was used to assess the effects of lactucin on cell cycle phase distribution. The results indicate that lactucin induced potent, time and dose-dependent antitumor effects. The microscopic techniques showed that lactucin induced characteristic features of apoptosis including cell shrinkage, membrane blebbing, appearance of vacuoles, swelling of mitochondria and endoplasmic reticulum. Viable cells are stained green, early apoptotic cells are stained yellow, while as late apoptotic cells are stained reddish orange. Flow cytometry revealed that lactucin induced sub-G1 cell cycle arrest.

Video Clips of Methodology:

Phase contrast microscopy:

One   Full Screen   Alternate

Two   Full Screen   Alternate

Three   Full Screen   Alternate

Four   Full Screen   Alternate

Five   Full Screen   Alternate

Six   Full Screen   Alternate

Seven   Full Screen   Alternate

Eight   Full Screen   Alternate

Nine   Full Screen   Alternate

Downloads

Download data is not yet available.
Abstract
1759
Download
910 Read
994

References

Bischoff TA, Kelley CJ, Karchesy Y, Laurantos M, Nguyen-Dinh P, Arefi AG. Antimalarial activity of lactucin and lactucopicrin: Sesquiterpene i from Cichorium intybus L. J Ethnopharmacol. 2004; 95: 45557.

Chadwick M1, Trewin H, Gawthrop F, Wagstaff C. Sesquiterpenoids lactones: Benefits to plants and people. Int J Mol Sci. 2013; 14: 12780805.

Dall'Acqua S, Viola G, Giorgetti M, Loi MC, Innocenti G. Two new sesquiterpene lactones from the leaves of Laurus nobilis. Chem Pharm Bull. 2006; 54: 118789.

Hengartner MO. The biochemistry of apoptosis. Nature 2000; 407: 770-76.

Hoffbrand AV, Moss PAH, Pettit JE. Essential haematology. Blackwell. 5th edn. 2006.

Hutter JJ. Childhood leukemia. Pediatr Rev/American Academy of Pediatrics. 2010; 31: 23441.

Kerr JF, Wyllie AH, Currie AR. Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer. 1972; 26: 239-57.

Kreuger MR, Grootjans S, Biavatti MW, Vandenabeele P, D'Herde K. Sesquiterpene lactones as drugs with multiple targets in cancer treatment: Focus on parthenolide. Anticancer Drugs. 2012; 23: 883-96.

Ren YL, Zhou YW, Chen XZ, Ye YH. Discovery, structural determination and anti-cancer activities of lactucin-like guaianolides. Lett Drug Des Discov. 2005; 2: 444.

Vardiman JW, Thiele J, Arber DA, Brunning RD, Borowitz MJ, Porwit A, Harris NL, Le Beau MM, Hellström-Lindberg E, Tefferi A, Bloomfield CD. The 2008 revision of the World Health Organization (WHO) classification of myeloid neoplasms and acute leukemia: Rationale and important changes. Blood 2009; 114: 93751.

Weso?owska A, Nikiforuk A, Michalska K, Kisiel W, Chojnacka-Wójcik E. Analgesic and sedative activities of lactucin and some lactucin-Like guaianolides in mice. J Ethnopharmacol. 2006; 107: 25458.

World Health Organization. World Cancer Report. 2014.

Published

2016-04-15

How to Cite

Zhang, F.-H., Y.-L. Yan, Y. Wang, and Z. Liu. “Lactucin Induces Potent Anti-Cancer Effects in HL-60 Human Leukemia Cancer Cells by Inducing Apoptosis and Sub-G1 Cell Cycle Arrest”. Bangladesh Journal of Pharmacology, vol. 11, no. 2, Apr. 2016, pp. 478-84, doi:10.3329/bjp.v11i2.26729.

Issue

Section

Research Articles