Hepatoprotective effect of Helicanthus elastica
Keywords:Helicanthus elastica, Hepatoprotective
Search for medicinal plants to treat liver disorders is an important research topic on herbs. Acute toxicity study is a prerequisite for safety and dose fixation for further pharmacological actions. In the present study, aqueous and 95% ethanolic extract of whole plant of Helicanthus elastica were subjected to acute oral toxicity. The aqueous and ethanolic extract revealed no observable changes in the rats up to the dose level of 2,000 mg /kg body weight. The extracts were then screened for paracetamol-induced hepatic injury at dose levels of 200 and 400 mg/kg body weight (1/10 and 1/5 LD50 based on toxicity study). The aqueous extract of whole plant of H. elastica was found to produce significant (p<0.05) reversal of the paracetamol-induced changes in the measured biochemical and histopathological parameters at lower dose of 200 mg/kg which was found to be better than ethanol extract at the same dose level.
Video Clip of Methodology:
Aleykutty NA, Srinivasan KK, Rao GP, Keshavamurthy KR, Udupa AL. Study of the diuretic activity of Helicanthes elastica. Fitoterapia 1991; 62: 439-40.
Bauer I, Vollmar B, Jaeschke H, Rensing H, Kraemer T, Larsen R, Bauer M. Transcriptional activation of heme oxygenase-1 and its functional significance in acetaminophen-induced hepatitis and hepatocellular injury in the rat. J Hepatol. 2000; 33: 395.
Bergmeyer HU, Horder M, Rej R. IFCC methods for the measurements of catalytic concentrations of enzymes. J Clin Chem Biochem. 1986; 24: 481.
Bussing A. Biological and pharmacological properties of Viscum album L. from tissue flask to man. In: Mistletoe: The genus Viscum. Büssing A. (ed). The Netherlands, The Gordon and Breach Publishing Group, 2004, p 123.
Desai SN, Patel DK, Devkar RV, Patel PV, Ramachandran AV. Hepatoprotective potential of polyphenol rich extract of Murraya koenigii L: An in vivo study. Food Chem Toxicol. 2012; 50: 310.
Eoin W, Jiang HW, Paul R, Qiong DW, Elaine K, David B. Role of taurine in preventing acetaminophen-induced hepatic injury in the rat. Am J Physiol Gastrointest Liver Physiol. 2007; 280: 1274.
Garber CC. Jendrassik-Grof analysis for total and direct bilirubin in serum with a centrifugal analyzer. Clin Chem. 1981; 27: 1410-16.
Guven K, Mehmet D, Ahmet N, Zafer Y, Rami L, Ahmet K, Tolunay K, Hasan K, Sadik S. Effects of erdosteine on acetaminophen-induced hepatotoxicity in rats. Toxicol Pathol. 2008; 36: 714.
Jadhav N, Patil CR, Chaudhari KB, Wagh JP, Surana SJ, Jadhav RB. Diuretic and natriuretic activity of two mistletoe species in rats. Phcog Res. 2010; 2: 50-57.
Kaviarasan S, Viswanathan P, Anuradha CV. Fenugreek seed (Trigonella foenum-graecum) polyphenols inhibit ethanol-induced collagen and lipid accumulation in rat liver. Cell Biol Toxicol. 2007; 23: 373.
Kirtikar KR, Basu BD. Indian medicinal plants. Vol. III. Dehradoon, M/S. Bishen Singh Mahendra Singh Pal, 1935, pp 2178-85.
Lubran MM. The measurement of total serum proteins by the Biuret method. Ann Clin Lab Sci. 1978; 8: 106.
Mary KT, Girija K, Ramadasan K. Partial purification of tumour reducing principle from Helicanthus elasticus (Fam. Loranthaceae). Cancer Lett. 1994; 81: 53-57.
Negi AS, Kumar JK, Luqman S, Shanker K, Gupta MM, Khanuja SPS. Recent advances in plant hepatoprotectives: A chemical and biological profile of some important leads. Med Res Rev. 2008; 28: 746.
Sangeetha B, Krishnakumari S. Tephrosia purpurea (Linn.) Pers: A folk medicinal plant ameliorates carbon tetrachloride-induced hepatic damage in rats. Int J Pharma Biosci. 2010; 1: 1.
Sanjeev H, Arun KB, Nitin M. Immunomodulatory activity of methanolic extracts of fruits and bark of Ficus glomerata Roxb. in mice and on human neutrophils. Indian J Pharmacol. 2013; 45: 130.
Schiodt FV, Rochling FA, Casey DL, Lee WM. Acetaminophen toxicity in an urban county hospital. N Engl J Med. 1997; 337: 1112.
Sunil Kumar KN, Saraswathy A, Amerjothy S, Balakrishna K. Phytochemical examination of compounds from mango mistletoeHelicanthus elastica (Desr.) Danser. IJC-B. 2015; 54B: 924-29.
Sunil Kumar KN, Saraswathy A, Amerjothy S, Ravishankar B. Antimicrobial potential of Helicanthus elastica (Desr.) Danser growing on mango (Mango mistletoe). J Trad Complem Med. 2014a; 4: 258-62.
Sunil Kumar KN, Saraswathy A, Amerjothy S, Susan T, Ravishankar B. Total phenol content and in vitro anti-oxidant potential of Helicanthus elastica (Desr.) Danser: A less explored Indian mango mistletoe. J Trad Complem Med. 2014b; 4: 285-88.
Sunil Kumar KN, Shakila R, Amerjothy S. Physicochemical evaluation, neutraceutical composition and HPLC-UV fingerprint of Helicanthus elastica (Desr.) Danser (Indian mango mistletoe). Int J Green Pharm. 2014c; 8: 175-79.
Sunil Kumar KN. Pharmacognostical, phytochemical and medicinal activity profile of Helicanthus elastica (Desr.) Danser (Mango mistletoe)Loranthaceae. PhD Thesis. Chennai. The University of Madras, 2011.
Tirtha G, Tapan KM, Mrinmay D, Anindya B, Deepak KD. In Vitro Anti-oxidant and hepatoprotective activity of ethanolic extract of Bacopa monnieri Linn. Aerial Parts, Iran J Pharmacol Ther. 2007; 6: 77.
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).