REN C-5312T, but not C-5434T, correlates to increase serum angiotensin I level in angiotensin II receptor blockers-treated hypertensive patients


  • Mohammad Saifur Rohman Department of Cardiology and Vascular Medicine, Faculty of Medicine, University of Brawijaya /Saiful Anwar General Hospital, Malang
  • Frastiqa Fahrany Biomedical Science, Faculty of Medicine, University of Brawijaya
  • Imama Maslahah Biomedical Science, Faculty of Medicine, University of Brawijaya
  • - Widodo Biology Department, Faculty of Mathematics and Sciences, University of Brawijaya
  • Mifetika Lukitasari Nursing Science, Faculty of Medicine, University of Brawijaya
  • Aditya Kurniawan Biology Department, Faculty of Mathematics and Sciences, University of Brawijaya
  • Agustina Tri Endharti Parasitology Department, Faculty of Medicine, University of Brawijaya



Angiotensin I receptor blocker, Angiotensin II receptor blocker, Hypertension, REN C-5312T, REN C-5434T


Renin distal enhancer plays a crucial role in regulating renin gene (REN) expression. REN C-5312T enhancer polymorphism increased enhancer activity. REN C-5434T was also identified which supposed to part of enhancer region. Therefore, this study aimed to investigate contribution of both C-5312T and C-5434T to serum angiotensin I in response to angiotensin II receptor blockers (ARB). C-5312T was identified in 46 hypertensive patients by using multiplex polymerase chain reaction (PCR), while C-5434T by using PCR-restiction fragment length of polymorphism (RFLP). Angiotensin I was measured using enzyme linked immunosorbent assay (ELISA). A significant difference of baseline angiotensin I was observed between -5312CC and -5312CT/TT (p=0.038) as well as the angiotensin I after 5 months of ARB treatment (p=0.008) in -5434CT/TT population, but not REN C-5434T. In conclusion, C-5312T resulted in increased mRNA renin level as consequence of higher enhancer activity not only at the baseline but also after 5 months ARB treatment.


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How to Cite

Rohman, M. S., F. Fahrany, I. Maslahah, .-. Widodo, M. Lukitasari, A. Kurniawan, and A. T. Endharti. “REN C-5312T, But Not C-5434T, Correlates to Increase Serum Angiotensin I Level in Angiotensin II Receptor Blockers-Treated Hypertensive Patients”. Bangladesh Journal of Pharmacology, vol. 11, no. 2, May 2016, pp. 552-7, doi:10.3329/bjp.v11i2.25818.



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