Daucosterol inhibits colon cancer growth by inducing apoptosis, inhibiting cell migration and invasion and targeting caspase signalling pathway


  • Gui-Qi Wang Department of Gastrointestinal Surgery, First Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei 050031
  • Jing-Feng Gu Department of Gastrointestinal Surgery, First Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei 050031
  • Ying-Chao Gao Department of Gastrointestinal Surgery, First Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei 050031
  • Yong-Jun Dai Department of Gastrointestinal Surgery, First Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei 050031




Apoptosis, Caspase signalling pathway, Cell migration, Colon cancer, Daucosterol, Invasion


The aim of the present investigation was to examine and demonstrate in detail the anti-cancer and apoptotic effects of daucosterol in human colon cancer cell line HCT-116. The effects of this compound on cell migration, cell invasion, cell cycle analysis and caspase signalling pathway were also studied. Cell viability was evaluated by MTT assay using different doses of the drug. In vitro wound healing assay was used to study cell migration. Flow cytometry was involved to examine cell apoptosis as well as cell cycle phase distribution. Daucosterol induced significant, dose-dependent as well as time-dependent cytotoxic effects with IC50 values of 26.6 and 47.3 µM at 24 and 48 hours time intervals respectively. The percent of cells that migrated decreased from 99% in case of untreated control to 84.2, 45.2, 39.5 and 14.4% in groups treated with 0, 5, 50, 75 and 100 µM of daucosterol respectively. Percentage of apoptotic cells increased from 2.5% in untreated control cells to 23.6, 46.9 and 74.2% in cells treated with 5, 50 and 100 µM dose of daucosterol respectively. Daucosterol at different doses induced cell cycle arrest at sub-G1 phase of the cell cycle.

Video Clip of Methodology:

Cell Migration Assay: 1 min   Full Screen   Alternate


Download data is not yet available.
900 Read


Ariazi EA, Satomi Y, Ellis MJ, Haag JD, Shi W, Sattler CA, Gould MN. Activation of the transforming growth factor beta signaling pathway and induction of cytostasis and apoptosis in mammary carcinomas treated with the anticancer agent perillyl alcohol. Cancer Res. 1999; 59: 1917 28.

de Gramont A, Figer A, Seymour M, Homerin M, Hmissi A, Cassidy J, Boni C, Cortes-Funes H, Cervantes A, Freyer G, Papamichael D, Le Bail N, Louvet C, Hendler D, de Braud F, Wilson C, Morvan F, Bonetti A.Leucovorin and fluorouracil with or without oxaliplatin as first-line treatment in advanced colorectal cancer. J Clin Oncol. 2000; 18: 293847.

Douillard JY, Siena S, Cassidy J, Tabernero J, Burkes R, Barugel M, Humblet Y, Bodoky G, Cunningham D, Jassem J, Rivera F, Kocákova I, Ruff P, B?asi?ska-Morawiec M, makal M, Canon JL, Rother M, Oliner KS, Wolf M, Gansert J. Randomized phase III trial of panitmumab with infusional fluorouracil, leucovorin, and oxaliplatin (FOLFOX4) versus FOLFOX4 alone as first-line treatment in patients with previously untreated metastatic colorectal cancer: The PRIME study. J Clin Oncol. 2010; 28: 4697705.

Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM. Estimates of world wide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 2010; 127: 2893-917.

Folprecht G, Grothey A, Alberts S, Raab HR, Kohne CH. Neoadjuvant treatment of unresectable colorectal liver metastases: Correlation between tumour response and resection rates. Ann Oncol. 2005; 16: 131119.

Friedl P, Wolf K. Tumour-cell invasion and migration: Diversity and escape mechanisms. Nat Rev Cancer. 2003; 3: 362-74.

Lowe SW, Lin AW. Apoptosis in cancer. Carcinogenesis 2000; 21: 485-95.

Miquel K, Pradines A, Tercé F, Selmi S, Favre G. Farnesol and geranylgeraniol induce actin cytoskeleton disorganization and apoptosis in A549 lung adenocarcinoma cells. Biochem Biophys Res Commun. 1996; 225: 86976.

Rodriguez-Nieto S, Zhivotovsky B. Role of alterations in the apoptotic machinery in sensitivity of cancer cells to treatment. Curr Pharm Des. 2006; 12: 4411-25.

Sakagami H1, Kuribayashi N, Iida M, Sakagami T, Takeda M, Fukuchi K, Gomi K, Ohata H, Momose K, Kawazoe Y. nduction of DNA fragmentation by tannin- and lignin-related substances. Anticancer Res. 1995; 15: 212128.

Saltz LB, Clarke S, Díaz-Rubio E, Scheithauer W, Figer A, Wong R, Koski S, Lichinitser M, Yang TS, Rivera F, Couture F, Sirzén F, Cassidy J. Bevacizumab in combination with oxaliplatin-based chemotherapy as first-line therapy in metastatic colorectal cancer: A randomized phase III study. J Clin Oncol. 2008; 26: 201319.

Van Cutsem E, Oliveira J. Advanced colorectal cancer: ESMO clinical recommendations for diagnosis, treatment and follow-up. Ann Oncol. 2009; 20: 6163.

Yamaguchi H, Wyckoff J, Condeelis J. Cell migration in tumors. Curr Opin Cell Biol. 2005; 17: 559-64.

Yoon Y, Kim YO, Lim NY, Jeon WK, Sung HJ. Shikonin, an ingredient of Lithospermum erythrorhizon induced apoptosis in HL60 human premyelocytic leukemia cell line. Planta Med. 1999; 65: 53235.



How to Cite

Wang, G.-Q., J.-F. Gu, Y.-C. Gao, and Y.-J. Dai. “Daucosterol Inhibits Colon Cancer Growth by Inducing Apoptosis, Inhibiting Cell Migration and Invasion and Targeting Caspase Signalling Pathway”. Bangladesh Journal of Pharmacology, vol. 11, no. 2, Mar. 2016, pp. 395-01, doi:10.3329/bjp.v11i2.25754.



Research Articles