Chemical characterization and mode of action of Ligustrum lucidum flower essential oil against food-borne pathogenic bacteria

Authors

DOI:

https://doi.org/10.3329/bjp.v11i1.25487

Keywords:

Essential oil, Food-borne pathogenic bacteria, Ligustrum lucidum

Abstract

Current research analyzes the chemical composition of Ligustrum lucidum flower essential oil obtained by the hydrodistillation, and examines its anti-microbial mode of action against food-borne pathogenic bacteria. Gas chromatography-mass spectrometry analysis of the oil resulted in the determination of 44 different compounds, representing 85.2% of the total oil. The oil (1 mg/disc) showed significant antibacterial effect as diameters of inhibition zones (14.6 ± 0.2 19.7 ± 0.3 mm), as well as minimum inhibitory and minimum bactericidal concentrations values (2501000 and 2502000 µg/mL), respectively. Based on the susceptibility, L. lucidum flower oil revealed its mode of action on membrane integrity as confirmed by increased release of extracellular ATP (2.5 and 2.2 pg/mL), leakage of potassium ions (950 and 900 mM/L), loss of 260-nm absorbing materials (4.2 and 3.9 optical density), and increase in relative electrical conductivity (10.6 and 9.8%) against Staphylococcus aureus KCTC-1621 (Gram-positive) and Salmonella enterica ATCC-4731 (Gram-negative), respectively.

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MIC determination: 10 min 32 sec   Full Screen   Alternate

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Author Biographies

Vivek K. Bajpai, Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712749

Assistant Professor

Google Scholar Citation Index

Siddhartha Singh, Department of Botany, Dr. Hari Singh Gour University, Sagar 470003, Madhya Pradesh

Post-doc

Archana Mehta, Department of Botany, Dr. Hari Singh Gour University, Sagar 470003, Madhya Pradesh

Professor

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Published

2016-01-29

How to Cite

Bajpai, V. K., S. Singh, and A. Mehta. “Chemical Characterization and Mode of Action of Ligustrum Lucidum Flower Essential Oil Against Food-Borne Pathogenic Bacteria”. Bangladesh Journal of Pharmacology, vol. 11, no. 1, Jan. 2016, pp. 269-80, doi:10.3329/bjp.v11i1.25487.

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Section

Research Articles