Antispasmodic activity of Symplocos paniculata is mediated through opening of ATP-dependent K+ channel

  • Khalid Hussain Janbaz Faculty of Pharmacy, Bahauddin Zakariya University, Multan
  • Saba Akram Faculty of Pharmacy, Bahauddin Zakariya University, Multan
  • Fatima Saqib Faculty of Pharmacy, Bahauddin Zakariya University, Multan http://orcid.org/0000-0001-6242-7147
  • Mamoona Khalid Faculty of Pharmacy, Bahauddin Zakariya University, Multan
Keywords: Antispasmodic, ATP-dependent K channel, Symplocos paniculata

Abstract

Symplocos paniculata is a medicinal plant used by native healers to manage gastrointestinal ailments. The crude methanolic extract of S. paniculata was screened pharmacologically both in vitro and in vivo for the validation of its therapeutic potential. It suppressed the spontaneous activity of isolated rabbit jejunum preparations and also caused inhibition of the low K+ (20 mM)- induced spastic contractions in isolated rabbit jejunum preparations in a manner comparable to cromakalim. The relaxant effect was found to be blocked following glibenclamide exposure of the isolated tissue preparations similar to cromakalim, suggesting that observed response was likely to be mediated through opening of ATP dependent K+ channels. Following oral administration to mice provided protection against castor oil-induced diarrhea in a manner similar to loperamide. The plant material was found safe in toxicity study up to oral dose of 8 g/kg in mice.  Hence, present study provides a scientific basis for the vernacular use of S. paniculata in gastro-intestinal system.

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Author Biographies

Khalid Hussain Janbaz, Faculty of Pharmacy, Bahauddin Zakariya University, Multan

Professor and Dean

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Fatima Saqib, Faculty of Pharmacy, Bahauddin Zakariya University, Multan
Faculty of Pharmacy

References

Bolton TB. Mechanism of action of transmitters and other substances on smooth muscles. Physiol Rev. 1979; 59: 606-718.

Chopra RN, Nayar SL, Chopra IC. Symplocos paniculata. In: Glossary of Indian medicinal plants. New Delhi, Council of Scientific and Industrial Research, 1956, p 240.

Dymock W, Warden CJH, Hooper D. Pharmacographia Indica: A history of principal drugs of vegetable origin met within British India. Said HM (ed.). Vol. II. Karachi, Institute of Health and Tibbi Research, 1972, pp 102-486.

Empfield JR, Russell K, Trainor DA. Potassium channel openers: Therapeutic possibilities. Pharma News. 1995; 23-27.

Facciola SC. Symplocos paniculata. In: Cornucopia a source book of edible plants. California, Kampong Publications, 1990; p 183.

Hamilton AH, Weir SW, Weston TH. Comparison of the effects of BRL 34915 and verapamil on electrical and mechanical activity in rat portal vein. Brit J Pharmacol. 1986; 88: 103-11.

Harborne JB. Phytochemical methods: A guide to modern techniques of plant analysis. 3rd edi. New York, Chapman and Hall, 1998, pp 1-302.

Huxley A. The new RHS dictionary of gardening. New York, Macmillan Press, 1992, p 200.

Janbaz KH, Saqib F. Pharmacological evaluation of Dactyloctenium aegyptium, an indigenous plant used to manage gastrointestinal ailments. Bangladesh J Pharmacol. 2015; 10: 295-302.

Janbaz KH, Zaeem Ahsan M, Saqib F, Imran I, Zia-Ul-Haq M, Abid Rashid M. Scientific basis for use of Pyrus pashia Buch.-Ham. ex D. Don. fruit in gastrointestinal, respiratory and cardiovascular ailments. PLoS ONE. 2015a; 10, e0118605.

Janbaz KH, Akhtar T, Saqib F, Imran I, Haq MZU, Janaskul C, Feo VD, Moga M. Pharmacological justification of use of Solena heterophylla Lour. in gastrointestinal, respiratory and vascular disorders. J Translational Med. 2015b; 13: 134.

Janbaz KH, Arif J, Saqib F, Imran I, Ashraf M, Haq MZU, Jaafar HZ, Vincenzo DF. In vitro and in vivo validation of ethnopharmacological uses of methanol extract of Isodon rugosus Wall. ex Benth (Lamiaceae). BMC Complement Altern Med. 2014; 14: 14-71.

Janbaz KH, Latif MF, Saqib F, Imran I, Haq ZU, Feo VD. Pharmacological effects of Lactuca serriola L. in experimental model of gastrointestinal, respiratory and vascular ailments. Evid-Based Complement Altern Med. 2013; 2013: 1-9.

Joy PP, Thomas J, Mathew S, Skaria BP. Medicinal plants. In: Tropical horticulture. Vol 2. New Delhi, New India Publishing, 2001, pp 449-632.

Karaki H, Wiess G. Mini-review: Calcium release in smooth muscles. Life Sci. 1983; 42: 11112.

Kishii KI, Morimoto T, Nakajima N, Yamazaki K, Tsujitani M, Takayanagi I. Effects of LP-805, a novel vasorelaxant agent, a potassium channel opener, on rat thoracic aorta. Gen Pharmacol. 1992; 23: 347-53.

Kumar N, Jangwanan JS. Phytoconstituents of Symplocos paniculata (leaves). J Curr Chem Pharm Sci. 2012; 2: 76-80.

Nudrat ZS, Usha M. Medicinal and aromatic plants of India. Part I. Hyderabad, Ukaaz Publications, 2005, p 3.

Polunin O, Stainton A. Symplocos paniculata. In: Flowers of the Himalayas. Delhi, Oxford University Press, 1984, p 894.

Quest U. Potassium channel openers: Pharmacological and clinical aspects. Fundam Clin Pharmacol. 1992; 6: 27993.

Quest U, Cook NS. Moving together: K+ channel openers and ATP-sensitive K+ channels. Trend Pharmacol Sci. 1989; 10: 43135.

Saqib F, Janbaz KH, Latif MF, Gilani AH, Bashir S. Ethno- pharmacological studies on antispasmodic, bronchodilator and antiplatelet aggregation activities of Blepharis edulis Pers. Asian J Nat App Sci. 2012; 1: 33-45.

Semwal RB, Semwal DK, Semwal R, Singh R, Rawat MSM. Chemical constituents from the stem bark of Symplocos paniculata Thunb. with antimicrobial, analgesic and anti-inflammatory activities. J Ethnopharmacol. 2011; 135: 78-87.

Weston AH, Edwards G. Recent progress in potassium channel opener pharmacology. Biochem Pharmacol. 1992; 43: 47-54.

Williamson EM, Okpako DT, Evans FJ. Selection, preparation and pharmacological evaluation of plant material. Chichester, John Wiley and Sons, 1998, pp 1523.

Published
2016-04-29
How to Cite
Janbaz, K., S. Akram, F. Saqib, and M. Khalid. “Antispasmodic Activity of Symplocos Paniculata Is Mediated through Opening of ATP-Dependent K+ Channel”. Bangladesh Journal of Pharmacology, Vol. 11, no. 2, Apr. 2016, pp. 495-00, doi:10.3329/bjp.v11i2.24667.
Section
Research Articles