Antifungal activity and brine shrimp toxicity assessment of Bulbine abyssinica used in the folk medicine in the Eastern Cape Province, South Africa

Authors

  • Cromwell Mwiti Kibiti Medicinal Plants and Economic Development (MPED) Research Center, Department of Botany, University of Fort Hare, Alice, 5700 http://orcid.org/0000-0002-3611-4427
  • Anthony Jide Afolayan Medicinal Plants and Economic Development (MPED) Research Center, Department of Botany, University of Fort Hare, Alice, 5700

DOI:

https://doi.org/10.3329/bjp.v11i2.24405

Keywords:

Antifungal, Brine shrimp, Bulbine abyssinica

Abstract

Bulbine abyssinica is widely used in folk medicine in Eastern Cape Province, South Africa. The aim of this study was to evaluate the antifungal and toxicity potentials of essential oil, acetone and aqueous extracts of this species using standard procedures and brine shrimp test, respectively. The results showed that the species was active against the growth of Microsporum canis, Microsporum gypseum and Trichophyton rubrum among the evaluated opportunistic fungi. The toxicity results showed that the lowest cysts hatching success was observed with the essential oil, then acetone extract, with aqueous extract exhibiting the highest hatching success. Based on the criterion of toxicity indices of the lethality test, all the plant fractions exhibited LD50 values greater than 1 mg/mL hence are non-toxic. These findings indicate that B. abyssinica is a good source of antifungal agents.

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Author Biographies

Cromwell Mwiti Kibiti, Medicinal Plants and Economic Development (MPED) Research Center, Department of Botany, University of Fort Hare, Alice, 5700

PhD student

Anthony Jide Afolayan, Medicinal Plants and Economic Development (MPED) Research Center, Department of Botany, University of Fort Hare, Alice, 5700

Research Professor

References

Afolayan AJ, Wintola OA. A survey of medicinal plants used in the treatment of dysentery in Amathole district municipality, South Africa. Pakistan J Bot. 2014; 46: 1685-92.

Bastos MLA, Lima MRF, Conserva LM, Andrade VS, Rocha EMM, Lemos RPL. Studies on the antimicrobial activity and brine shrimp toxicity of Zeyheria tuberculosa (Vell.) Bur. (Bignoniaceae) extracts and their main constituents. Ann Clin Microbiol Antimicrob. 2009.

Caldwell GS, Bentley MG, Olive PJW. The use of a brine shrimp (Artemia salina) bioassay to assess the toxicity of diatom extracts and short chain aldehydes. Toxicon 2003; 42: 30106.

Carballo JL, Hernandez-Inda ZL, Perez P, Garcia-Gravalos MD. A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products. BMC Biotechnol. 2002; 2: 17.

Chin-Hong PV. Infections in patients with diabetes mellitus: Importance of early recognition, treatment, and prevention. Infectious Diseases. 2006; 6: 72-81.

Council of Europe, European Pharmacopoeia Commission European Directorate for the Quality of Medicines and Healthcare. European Pharmacopoeia. 4th ed. Strasbourg, Council of Europe, 2002.

Drozdowska A, Drzewoski J. Mycoses in diabetes-difficult diagnostic and therapeutic problem. Review of literature. Diabetologia Do?wiadczalna Kliniczna. 2008; 8: 111.

Eckhard M, Lengler A, Liersch J, Bretzel RG, Mayser P. Fungal foot infections in patients with diabetes mellitus: Results of two independent investigations. Mycoses 2007; 50: 1419.

Holt RIG. Diagnosis, epidemiology and pathogenesis of diabetes mellitus: An update for psychiatrists. Brit J Psychiat. 2004; 184: 55-63.

Lalisan JA, Nuneza OM, Uy MM. Brine shrimp (Artemia salina) bioassay of the medicinal plant Pseudelephantopus spicatus from Iligan City, Philippines. Int Res J Biol Sci. 2014; 3: 47-50.

Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. Brine shrimp: A convenient general bioassay for active plant constituents. J Med Plants Res. 1982; 45: 3134.

Mishra NN, Prasad T, Sharma N, Paysi A, Prasad R, Gupta DK, Singh R. Pathogenicity and drug resistance in Candida albicans and other yeast species: A review. Acta Microbiol Immunol Hung. 2007; 54: 201-35.

Olaokun OO, McGaw LJ, Eloff JN, Naidoo V. Evaluation of the inhibition of carbohydrate hydrolyzing enzymes, antioxidant activity and polyphenolic content of extracts of ten African Ficus species (Moraceae) used traditionally to treat diabetes. BMC Complement Altern Med. 2013; 13: 94.

Otang WM, Grierson DS, Ndip RN. Antifungal activity of Arctotis arctotoides (L.f.) O. Hoffm. and Gasteria bicolor Haw. against opportunistic fungi associated with human immunodeficiency virus/acquired immunodeficiency syndrome. Pharmacogn Mag. 2012; 8: 135-40.

Otang WM, Grierson DS, Ndip RN. Assessment of potential toxicity of three South African medicinal plants using the brine shrimp (Artemia salina) assay. Afr J Pharm Pharmacol. 2013; 7: 1272-79.

Oyedemi SO, Bradley G, Afolayan AJ. Ethnobotanical survey of medicinal plants used for the management of diabetes mellitus in the Nkonkobe municipality of South Africa. J Med Plants Res. 2009; 3: 1040-44.

Pelka M, Danzl C, Distler W, Petschelt A. A new screening test toxicity testing of dental materials. J Dent. 2000; 28: 341-45.

Pooley E. A field guide to wild flowers of KwaZulu-Natal and the Eastern Region. Natal Flora Trust Publications, Durban, South Africa, 1998.

Prabuseenivasan S, Jayakumar M, Ignacimuthu S. In vitro antibacterial activity of some plant essential oils. BMC Complement Altern Med. 2006; 6: 39.

Sahgal G, Ramanathan S, Sasidharan S, Mordi MN, Ismail S, Mansor SM. Brine shrimp lethality and acute oral toxicity studies on Swietenia mahagoni (Linn.) Jacq. seed methanolic extract. Pharmacognosy Res. 2010; 2: 21520.

Sreejamole KL, Radhakrishnan CK. Antioxidant and cytotoxic activities of ethyl acetate extract of the Indian green mussel Perna viridis. Asian J Pharm Clin Res. 2013; 6: 197-201.

Syahmi ARM, Vijayarathna S, Sasidharan S, Latha LY, Kwan YP, Lau YL, Shin LN, Chen Y. Acute oral toxicity and brine shrimp lethality of Elaeis guineensis Jacq., (oil palm leaf) methanol extract. Molecules 2010; 15: 8111-21.

Ullah R, Bakht J, Shafi M. Antimicrobial and anti-oxidant potential of Periploca hydaspidis. Bangladesh J Pharmacol. 2015; 10: 645-51.

Vasconcelos V, Azevedo J, Silva M, Ramos V. Effects of marine toxins on the reproduction and early stages development of aquatic organisms. Mar Drugs. 2010; 8: 59-79.

Wanjohi JM, Yenesew A, Midiwo JO, Heydenreich M, Peter MG, Dreyer M, Reichert M, Bringmann G. Three dimeric anthracene derivatives from the fruits of Bulbine abyssinica. Tetrahedron 2005; 61: 2667-74.

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Published

2016-04-14

How to Cite

Kibiti, C. M., and A. J. Afolayan. “Antifungal Activity and Brine Shrimp Toxicity Assessment of Bulbine Abyssinica Used in the Folk Medicine in the Eastern Cape Province, South Africa”. Bangladesh Journal of Pharmacology, vol. 11, no. 2, Apr. 2016, pp. 469-77, doi:10.3329/bjp.v11i2.24405.

Issue

Vol. 11 No. 2 (2016)

Section

Research Articles

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