Anti-diabetic and anti-oxidant status of Loranthus pulverulentus obtained from two different hosts

Authors

  • Sairah Hafeez Kamran University College of Pharmacy, University of the Punjab, Allama Iqbal Campus, Lahore 54000 http://orcid.org/0000-0002-3526-4132
  • Mobasher Ahmad University College of Pharmacy, University of the Punjab, Allama Iqbal Campus, Lahore 54000
  • Durre Shahwar Department of Chemistry, Government College University, Lahore 54000
  • Muhammad Ajaib Department of Botany, Government College University, Lahore 54000

DOI:

https://doi.org/10.3329/bjp.v11i1.24056

Keywords:

Anti-diabetic, Anti-oxidant, Dalbergia sissoo, Loranthus pulverulentus, Populus nigra

Abstract

Leaves of Loranthus pulverulentus have been used ethnically in Pakistan for controlling blood glucose levels. The present study was designed to investigate the anti-diabetic and anti-oxidant potential of the methanolic extracted fractions of L. pulverulentus obtained from two different hosts viz. Dalbergia sissoo and Populus nigra. The acute study demonstrated that L. pulverulentus (D. sissoo) leaves possess significant blood glucose lowering effect whereas L. pulverulentus (P. nigra) was didnt significantly decreased blood glucose levels. The n-butanol fraction of L. pulverulentus (D. sissoo) also significantly increased in vivo total anti-oxidant activity on eighth day after treatment and blood glucose levels were significantly reduced (%46.7) when compared to the control diabetic group (%?198.1) and metformin treated group (%?25.9). Leaves of L. pulverulentus (D. sissoo) have good margin of safety as therapeutic index is 3.5. This study provides scientific evidence that leaves of L. pulverulentus (D. sissoo) possess potential anti-diabetic activity whereas L. pulverulentus (P. nigra) was not found effective in diabetic illness.

Downloads

Download data is not yet available.
Abstract
1282
Download
994 Read
828

Author Biography

Sairah Hafeez Kamran, University College of Pharmacy, University of the Punjab, Allama Iqbal Campus, Lahore 54000

Lecturer

References

Adesina SK, llloh HC, Johnny ll, Jacobs lE. African mistletoes (Loranthaceae), ethnopharmacology, chemistry, medicinal values: An update. Afr J Tradit Complement Altern Med, 2013; 10: 161-70.

Ajaib M, Khan Z, Khan M, Wahab M. Ethnobotanical studies on useful shrubs of district Kotli, Azad Jammu & Kashmir, Pakistan. Pakistan J Bot. 2010; 42: 1407-15.

Evans WC. Trease and Evans pharmacognosy. Elsevier Health Sciences, 2009.

Gee J, Johnson I. Interactions between hemolytic saponins, bile salts and small intestinal mucosa in the rat. J Nutr. 1988; 118: 1391-97.

GöktürkBaydar N, Özkan G, Ya?ar S. Evaluation of antiradical and anti-oxidant potential of grape extracts. Food Control. 2007; 18: 1131-36.

Ishtiaq S, Ahmad M, Hanif U, Akbar S, Kamran SH. Phytochemical and in vitro anti-oxidant evaluation of different fractions of Amaranthus graezians subsp. Silvestris (Vill.) Brenan. Asian Pac J Trop Med. 2014; 7: S342-47

Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for measurement of antioxidant activity in human fluids. J Clin Pathol. 2001; 54: 356-61.

Lenzen S. The mechanism of alloxan- and streptozocin-induced diabetes. Diabetologica 2008; 51: 216-26.

Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol. 1983; 54: 275-87.

OECD. 420: Acute oral toxicity-fixed dose procedure. OECD guidelines for testing of chemicals, 2001, pp 1-14.

ÖnayUçar E, Karagöz A, Arda N. Anti-oxidant activity of Viscum album ssp. Album. Fitoterapia 2006; 77: 556-60.

Raza MA, Kausar R, Rana FA, Danish M, Shahwar D, Anwar F. Lorathus pulveulentus: A potent source of natural anti-oxidants and alternative medicine. J Chem. 2013; 2013.

Sabu M, Kuttan R. Anti-diabetic activity of medicinal plants and its relationship with their anti-oxidant property. J Ethnopharmacol. 2002; 81: 95-102.

Singleton VL, Orthofer R, Lameula-Raventos RM. Analysis of total phenols and other oxidation substrates and anti-oxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999; 299: 152-78.

Stahl E. Thin-layer chromatography. A laboratory handbook. 2nd ed. Heidelberg, Springer-Verlag Berlin, 1967, pp 855-904.

Subhan F, Abbas M, Rauf K, Baseer A. Anti-GIT motility, toxicological and phytochemical studies on Bacopa monnieri. Pharmacology Online. 2010; 3: 937-95.

Published

2016-01-17

How to Cite

Kamran, S. H., M. Ahmad, D. Shahwar, and M. Ajaib. “Anti-Diabetic and Anti-Oxidant Status of Loranthus Pulverulentus Obtained from Two Different Hosts”. Bangladesh Journal of Pharmacology, vol. 11, no. 1, Jan. 2016, pp. 181-9, doi:10.3329/bjp.v11i1.24056.

Issue

Section

Research Articles