Determination of immune response against alum-precipitated fowl cholera vaccine in the quail, <i>Coturnix japonica</i>

Authors

  • MT Iqbal Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh
  • MH Haque Department of Animal Husbandry and Veterinary Science, University of Rajshahi, Rajshahi
  • S Sarker Department of Animal Husbandry and Veterinary Science, University of Rajshahi, Rajshahi
  • MA Islam Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh
  • KA Choudhury Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh

DOI:

https://doi.org/10.3329/ujzru.v29i1.9467

Keywords:

Fowl cholera, alum-precipitated vaccine, immune response, PHA test, quail

Abstract

The immune response of a formalin-inactivated alum-precipitated fowl cholera vaccine (FCV) was evaluated in quails, Coturnix japonica (Order: Galliformes and Family: Mimidae). All quails, irrespective of sex and 8-weeks-old were immunized with 5×107CFU/ml/quail subcutaneously (SC) and intramuscularly (IM). A Booster immunization was given with similar dose and routes at 15 days after primary immunization in groups A and B while group C served as unimmunized control. Pre-immunized sera were collected from all groups of birds to assay the primary antibody levels in them. Sera of the immunized and control quails were collected at 15 and 30 days post immunization. The degree of immunity produced in each group of quails following primary and secondary immunizations were determined by measuring their serum antibody titres using passive haemagglutination assay (PHA) test. The level of antibody was significantly increased both primary and booster immunization in immunized quails as determined by PHA titres. Two weeks after final immunization, the quails were challenged with a virulent isolate of fowl cholera and immunized quails conferred 100% protection while all the control quails were dead within 10 days post challenge.  

Key words: Fowl cholera; alum-precipitated vaccine; immune response; PHA test; quail

DOI: http://dx.doi.org/10.3329/ujzru.v29i1.9467

UJZRU 2010; 29(1): 57-59

Downloads

Download data is not yet available.
Abstract
98
PDF
81

Downloads

How to Cite

Iqbal, M., Haque, M., Sarker, S., Islam, M., & Choudhury, K. (2012). Determination of immune response against alum-precipitated fowl cholera vaccine in the quail, <i>Coturnix japonica</i>. University Journal of Zoology, Rajshahi University, 29(1), 57–59. https://doi.org/10.3329/ujzru.v29i1.9467

Issue

Section

Articles