Fertility of cryopreserved common carp (<i>Cyprinus carpio</i>) spermatozoa

Authors

  • M Sultana Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh
  • M Nahiduzzaman Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh
  • MM Hassan Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh
  • MUH Khanam Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh
  • MAR Hossain Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh

DOI:

https://doi.org/10.3329/ujzru.v28i0.5287

Keywords:

Cyprinus carpio, spermatozoa, cryopreservation, cryoprotectant, cryodiluent, fertility

Abstract

The study was aimed at standardization of cryopreservation protocol for the common
carp, (Cyprinus carpio) spermatozoa for using in the artificial propagation. Three extenders
(Alsever’s solution, egg-yolk citrate and 0.9% NaCl) were combined with three cryoprotectants
(ethanol, methanol and DMSO). Two-step freezing protocol (5°C to -4°C at a rate of 4°C per minute
then -4°C to -80°C at a rate of 10°C per minute and finally held for 10 min) was carried out in a
computer-controlled freezer where 0.25 ml straws containing spermatozoa were stored in a liquid
nitrogen container at -196°C. Alsever’s solution with ethanol, Alsever’s solution with methanol and
egg yolk citrate with DMSO gave better motility (%) in both pre-freeze (93.33±1.05, 90±1.83 and
88.33±2.11, respectively) and post-thaw (80±4.28, 74.17±3.96 and 76.67±4.59, respectively)
periods whereas, 10% cryoprotectant gave the best motility during post-thaw period. Considering
both pre-freeze and post-thaw motilities of the common carp spermatozoa, 1:9 was the best ratio
for the milt and cryodiluents. The egg yolk citrate with DMSO yielded the best fertilization
(37.67±1.76%) and hatching (28.67±1.85%) compared to other combinations under study.

Key words: Cyprinus carpio; spermatozoa; cryopreservation; cryoprotectant; cryodiluent; fertility.

DOI: 10.3329/ujzru.v28i0.5287

Univ. j. zool. Rajshahi Univ. Vol. 28, 2010 pp. 51-55

Downloads

Download data is not yet available.
Abstract
717
PDF
1169

Downloads

How to Cite

Sultana, M., Nahiduzzaman, M., Hassan, M., Khanam, M., & Hossain, M. (2010). Fertility of cryopreserved common carp (<i>Cyprinus carpio</i>) spermatozoa. University Journal of Zoology, Rajshahi University, 28, 51–55. https://doi.org/10.3329/ujzru.v28i0.5287

Issue

Section

Articles