Comparative Antioxidant Potential of Different Extracts of <i>Celastrus paniculatus</i> Willd. Seed

  • Fatema Tuz Zohera Department of Pharmacy, Jahangirnagar University
  • Md Razibul Habib Lecturer, Dept. of Pharmacy, International Islamic University, Chittagong
  • Mohammad Zafar Imam Department of Pharmacy, Stamford University Bangladesh
  • Md Ehsanul Hoque Mazumder Discipline of Biomedical Science, The University of Sydney
  • Md Sohel Rana Department of Pharmacy, Jahangirnagar University, Bangladesh
Keywords: Celastrus paniculatus, antioxidant, DPPH, NO scavenging, CUPRAC, ROS

Abstract

The objective of the present study was to evaluate the comparative antioxidant potential of methanol, ethyl acetate, pet ether and water extracts of Celastrus paniculatus seed. Antioxidant activity was evaluated by using total phenol and flavonoid content determination assays, total antioxidant capacity, 1,1-diphenyl-2- picryl-hydrazil (DPPH) free radical assay, Reducing power assessment, Nitric oxide (NO) scavenging assay and Cupric ion reducing capacity assay (CUPRAC method). The extracts showed moderate antioxidant activity in a dose dependent manner. The extracts were found to contain phenolics and flavonoid compounds. In DPPH radical scavenging assay, ethyl acetate extract had the lowest IC50 value (585.58μg/ml) compared to ascorbic acid. In nitric oxide scavenging assay IC50 value was found to be 122.99μg/ml, 320.54μg/ml, 601.81μg/ml and 206.37μg/ml respectively for the Water, Methanol, Ethyl Acetate and Pet Ether extracts compared to 6.83μg/ml which was the IC50 value for the reference ascorbic acid. The extracts also showed good reducing power. The results of the present study indicate that the extracts possesses significant antioxidant potential of which ethyl acetate extract is the most promising one and possess highest antioxidant potential.

Key Words: Celastrus paniculatus; antioxidant; DPPH; NO scavenging; CUPRAC; ROS.

DOI: 10.3329/sjps.v3i1.6802

S. J. Pharm. Sci. 3(1): 68-74

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How to Cite
Zohera, F., Habib, M., Imam, M., Mazumder, M., & Rana, M. (1). Comparative Antioxidant Potential of Different Extracts of <i>Celastrus paniculatus</i&gt; Willd. Seed. Stamford Journal of Pharmaceutical Sciences, 3(1), 68-74. https://doi.org/10.3329/sjps.v3i1.6802
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Research Articles