Infrared spectroscopic characterization, free radical scavenging and cytotoxic evaluation of chitosan extracted from <i>Penaeus monodon</i> shells
AbstractChitosans are naturally occurring biologically safe and non-toxic polymer of polysaccharides. In the present
study, Chitosan [(1-4) 2-amino-2-deoxy-β-D-glucan] was extracted from the exoskeleton of Black Tiger
(Penaeus monodon) shrimp shells by alkaline deacetylation of chitin. Three different chitosan extracts, coded
as Chito A (3.33% w/w), Chito B (4.01% w/w) and Chito C (3.45% w/w) were extracted using 3%, 4% and
5% w/v concentrations of HCl as decalcifiers respectively. Physicochemical properties such as appearance,
odor, insolubility and pH of all the extracts were found to comply with the compendial specifications of
pharmaceutical grade chitosan. Qualitative identification of the samples was carried out using Infrared (IR)
spectroscopy. Major peaks of the extracts matched with reference IR spectrum of standard chitosan.
Extracted chitosans were found lacking cytotoxicity in brine shrimp lethality bioassay. All the extracts showed
strong antioxidant activity in DPPH free radical scavenging assay and the IC50 values were found to be 37 ± 2
μg/mL (Chito A), 35 ± 1 μg/mL (Chito B) and 30 ± 2 μg/mL (Chito C) while the standard antioxidant Quercetin
showed an IC50 value of 15 ± 2 μg/mL.
Key words: Chitosan; Black Tiger (Penaeus monodon) shrimp shells; deacetylation of chitin; IR
spectroscopy; cytotoxicity; DPPH; free radical scavenging.
Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 27-30
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