Antioxidant, analgesic and cytotoxic activity of <i>Michelia champaca</i> Linn. Leaf
AbstractMichelia champaca (Magnoliaceae) is a large medicinal plant which is traditionally used against a number of
diseases including inflammatory conditions. In the present study crude methanol extract of M. champaca leaf
was investigated for possible antioxidant, analgesic and cytotoxic activity. The extract showed remarkable
antioxidant activity in DPPH radical scavenging activity, nitric oxide scavenging activity and total antioxidant
capacity assays. In both DPPH radical and NO scavenging assay, the extract exhibited strong antioxidant
activity and the IC50 values in DPPH radical scavenging and NO scavenging assays were found to be 30.07
μg/ml and 15.42 μg/ml, respectively while the IC50 values of ascorbic acid were 12.5 μg/ml and 4.07 μg/ml,
respectively. Total antioxidant activity of the extract increased in a dose dependent manner. Analgesic activity
of the crude extract was evaluated using acetic acid-induced writhing model of pain in mice. The crude extract
at 200 mg/kg and 400 mg/kg b.w doses displayed significant (p < 0.001) reduction in acetic acid induced
writhing in mice with a maximum effect of 68.7% reduction at 400 mg/kg b.w. which is comparable to the
standard, diclofenac sodium (78.2%). The extract was also investigated for cytotoxic potential using Brine
Shrimp lethality bioassay. In this bioassay the extract showed significant toxicity to Brine Shrimp nauplii with
the LC50 value of 11.22 μg/ml. Results of the present study suggest that M. champaca leaf extract possesses
strong antioxidant, analgesic and cytotoxic activity.
Key words: Michelia champaca; Magnoliaceae; Antioxidant; Analgesic; Cytotoxicity.
Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 1-7
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