In-vitro Antioxidant and Cytotoxicity Studies of <i>Annona squamosa</i> Linn.
Abstractn-Hexane, chloroform and methanol soluble extracts of the leaves of Annona squamosa were screened for
their possible antioxidant activitiy by DPPH free radical scavenging and cytotoxicity by brine shrimp lethality
bioassay. In DPPH radical scavenging assay, methanol soluble extract was found to be the most potent with
an IC50 value of 103.5 μg/ml. The amount of total phenolics was also found to the highest in the methanol
soluble extract (283.16 ± 8.90 mg/g), followed by chloroform soluble extract (216.90 ± 4.48 mg/g). Here BHT
and ascorbic acid were used as standards with IC50 values 8.2 μg/ml and 25 μg/ml respectively. In the brine
shrimp lethality bioassay, the most significant cytotoxicity was observed with chloroform soluble extract with
an LC50 of 4.16 μg/ml where vincristine sulphate was used as standard (LC50 0.29 μg/ml).
Key Words: Annona squamosa; DPPH; Folin-Ciocalteu Reagent; Phenolic Contents; Brine Shrimp
Stamford Journal of Pharmaceutical Sciences Vol.2(1) 2009: 32-36
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