In-vitro Antioxidant and Cytotoxicity Studies of <i>Annona squamosa</i> Linn.

Authors

  • Israt Jahan Biva Department of Pharmacy, Stamford University Bangladesh
  • Md Mynol Islam Vhuiyan Department of Pharmacy, North South University
  • Moni Rani Saha Department of Pharmacy, North South University
  • Muhammad Shahidul Islam Department of Pharmacy, North South University
  • Shammy Sarwar Department of Pharmacy, North South University

DOI:

https://doi.org/10.3329/sjps.v2i1.5813

Keywords:

Annona squamosa, DPPH, Folin-Ciocalteu Reagent, Phenolic Contents, Brine Shrimp

Abstract

n-Hexane, chloroform and methanol soluble extracts of the leaves of Annona squamosa were screened for
their possible antioxidant activitiy by DPPH free radical scavenging and cytotoxicity by brine shrimp lethality
bioassay. In DPPH radical scavenging assay, methanol soluble extract was found to be the most potent with
an IC50 value of 103.5 μg/ml. The amount of total phenolics was also found to the highest in the methanol
soluble extract (283.16 ± 8.90 mg/g), followed by chloroform soluble extract (216.90 ± 4.48 mg/g). Here BHT
and ascorbic acid were used as standards with IC50 values 8.2 μg/ml and 25 μg/ml respectively. In the brine
shrimp lethality bioassay, the most significant cytotoxicity was observed with chloroform soluble extract with
an LC50 of 4.16 μg/ml where vincristine sulphate was used as standard (LC50 0.29 μg/ml).

Key Words: Annona squamosa; DPPH; Folin-Ciocalteu Reagent; Phenolic Contents; Brine Shrimp

DOI: 10.3329/sjps.v2i1.5813

Stamford Journal of Pharmaceutical Sciences Vol.2(1) 2009: 32-36

Downloads

Download data is not yet available.
Abstract
793
PDF
918

Downloads

How to Cite

Biva, I. J., Vhuiyan, M. M. I., Saha, M. R., Islam, M. S., & Sarwar, S. (2010). In-vitro Antioxidant and Cytotoxicity Studies of <i>Annona squamosa</i> Linn. Stamford Journal of Pharmaceutical Sciences, 2(1), 32–36. https://doi.org/10.3329/sjps.v2i1.5813

Issue

Vol. 2 No. 1 (2009)

Section

Research Articles

License

As a condition of publication, all authors must transfer copyright to the Department of Pharmacy. Manuscripts submitted under multiple authorship are reviewed on the assumption that all listed authors concur in the submission, and that the final version of the manuscript has been seen and approved by them.

Each author must agree to this statement

Authorship: This manuscript is the original work of the authors, each of whom has read and approved of the work. Each author satisfied the requirements contained in 'Author Guidelines' having participated sufficiently in the work to take public responsibility for the content. This participation includes:

  • Conception or design of the study, or analysis and interpretation of data, or both
  • Drafting the article or revising it for critically important intellectual content
  • Approval of the final 'to be published' version

All authors must take responsibility for the integrity of the work. Participating solely in the collection of data does not justify authorship.

Prior publication: This work is not currently under consideration by any other journal. Information about prior publication of any part of this work, or inclusion of patients detailed herein in any other work, has been provided in the cover letter.

Conflict of interest: Details of any financial or other relationship between any author and any other party that may lead to a conflict of interest with the subject or any materials mentioned in this article have been disclosed in the cover letter.