Cytotoxicity and Antifungal Activities of Root Bark of <i>Calotropis gigantea</i>
AbstractIn this study, methanol extract from the root bark of Calotropis gigantea L. and its petroleum ether (40°C-
60°C), chloroform and ethyl acetate soluble fractions were tested for their cytotoxic activity against brine
shrimp nauplii (Artemia salina, Leach) and for antifungal activity against Aspergillus flavus, Aspergillus niger,
Penicillium sp and Trichoderma harzianum. Thin layer chromatography (TLC) screening showed that
methanol extract and its different fractions contained different type compounds such as steroid, terpene,
glycoside, heterocyclic and flavonoid. In brine shrimp lethality bioassay, it was found that chloroform fraction
was highly cytotoxic (LD50 14.72 μg/ml) among the tested samples. Though methanol extract and ethyl
acetate fraction have no activity against all the tested fungi but petroleum ether and chloroform fractions
showed potent activity against Aspergillus niger, Penicillium sp, Trichoderma harzianum and Aspergillus
niger, Trichoderma harzianum, respectively, in antifungal activity test.
Key words: Calotropis gigantea; Methanol extract; Antifungal activity; Cytotoxicty.
Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 38-41
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