Studies on microscopic technique and culture on Sabouraud's dextrose agar medium for diagnosis of dermatophytes infection

Authors

  • Md Abul Abul Hossain Khan Associate Professor & Head, Department of Microbiology, Khwaja Yunus Ali Medical College, Enayetpur-Sirajgonj.
  • S M Atique Hasan Khan Assistant Professor, Department of Dermatology and Venerology, Khwaja Yunus Ali Medical College, Enayetpur-Sirajgonj.
  • Mahbub Rashid Sarker Pathologist, Department of Laboratory Services, Khwaja Yunus Ali Medical College, Enayetpur-Sirajgonj
  • Rubiyat Farzana Hussain Assistant Professor, Department of Community Medicine, Khwaja Yunus Ali Medical College, Enayetpur-Sirajgonj
  • Mejbah Uddin Ahmed Associate Professor, Department of Microbiology, Enam Medical College, Saver-Dhaka
  • Yasmin Joarder Assistant Professor, Department of Dermatology and Venerology, Northern International Medical College, Dhanmondi-Dhaka
  • Md Bulbul Hasan Assistant Professor, Department of Microbiology, Rajshahi Medical College, Rajshahi

DOI:

https://doi.org/10.3329/kyamcj.v3i1.13658

Keywords:

Dermatophytes, Sabouraud's dextrose agar medium, Hyphae

Abstract

Dermatophytoses (a fungal infection of the skin, hair and nail, usually caused by dermatophytes) constitutes an important public health problem because of its high prevalence and associated morbidity but not life-threatening. Three genera of dermatophytes are recognized based on the site and pattern of fungal invasion. Dermatophytes are the predominant pathogenic mould, but yeasts (especially Candida albicans) and non-dermatophytic moulds may also be implicated. For accurate diagnosis of dermatophytoses requires microscopic demonstration and isolation and identification by culture. This study evaluates the usefulness of microscopic technique and culture for the isolation and identification of dermatophytes from clinical samples. Thirty samples were included in this study for detection of fungal elements by both methods but sensitivity of microscopic demonstration and culture were 60.0% and 66.7% respectively. As the sensitivity of microscopic demonstration (60.0%) is almost equal to the isolation and identification rate (66.7%), requires further evaluation in large scale as its ready to use format makes the application and microscopy much easier and faster.

DOI: http://dx.doi.org/10.3329/kyamcj.v3i1.13658

KYAMC Journal Vol. 3, No.-1, June 2012 pp.235-238

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Published

2013-02-05

How to Cite

Khan, M. A. A. H., Khan, S. M. A. H., Sarker, M. R., Hussain, R. F., Ahmed, M. U., Joarder, Y., & Hasan, M. B. (2013). Studies on microscopic technique and culture on Sabouraud’s dextrose agar medium for diagnosis of dermatophytes infection. KYAMC Journal, 3(1), 235–238. https://doi.org/10.3329/kyamcj.v3i1.13658

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Original Articles