16S rRNA Gene-targeted TTGE in Determining Diversity of Gut Microbiota during Acute Diarrhoea and Convalescence

Authors

  • Shirajum Monira Senior Scientist Enteric and Food Microbiology Laboratory Centre for Food and Waterborne Diseases icddr,b 68 Shaheed Tajuddin Ahmed Sarani Mohakhali, Dhaka 1212
  • Syeda Antara Shabnam icddr,b, GPO Box 128, Dhaka 1000
  • Nur Haque Alam icddr,b, GPO Box 128, Dhaka 1000
  • Hubert Ph. Endtz icddr,b, GPO Box 128, Dhaka 1000
  • Alejandro Cravioto icddr,b, GPO Box 128, Dhaka 1000
  • Munirul Alam icddr,b, GPO Box 128, Dhaka 1000

DOI:

https://doi.org/10.3329/jhpn.v30i3.12287

Keywords:

Child, Convalescence, Diarrhoea, Acute, Gut, Microbiota, 16S rRNA gene, Bangladesh

Abstract

The human gut microbiota play a vital role in health and nutrition but are greatly modified during severe  diarrhoea due to purging and pathogenic colonization. To understand the extent of loss during and after  diarrhoea, faecal samples collected from children (n=21) suffering from acute diarrhoea and from their  healthy siblings (n=9) were analyzed by 16S rRNA gene-targeted universal primer polymerase chain reaction  (PCR), followed by temporal temperature gradient gel electrophoresis (TTGE). The gut microbiota  decreased significantly as indicated by the number of TTGE bands at day 0 of acute diarrhoea [patients vs  healthy siblings: 11±0.9 vs 21.8±1.1 (mean±standard error), p<0.01]. The number of bands showed a steady  increase from day 1 to day 7; however, it remained significantly less than that in healthy siblings (15±0.9,  p<0.01). These results suggest that appropriate therapeutic and post-diarrhoeal nutritional intervention  might be beneficial for the early microbial restoration and recovery.

DOI: http://dx.doi.org/10.3329/jhpn.v30i3.12287

J HEALTH POPUL NUTR 2012 Sep;30(3):250-256

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Published

2012-10-20

How to Cite

Monira, S., Shabnam, S. A., Alam, N. H., Endtz, H. P., Cravioto, A., & Alam, M. (2012). 16S rRNA Gene-targeted TTGE in Determining Diversity of Gut Microbiota during Acute Diarrhoea and Convalescence. Journal of Health, Population and Nutrition, 30(3), 250–256. https://doi.org/10.3329/jhpn.v30i3.12287

Issue

Section

Original Papers