Jute endophyte Grammothele lineata SDL-CO-2015-1 produces an anticancer compound epothilone B

Authors

  • Mirza Ashikul Beg Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh
  • Sumaiya Binte Hannan Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh
  • - Rabeya Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh
  • Shafi Ahmad Talukder Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh
  • Aftab Uddin Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh
  • Shammi Akter Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh
  • Rifath Nehleen Reza Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh
  • Haseena Khan Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh
  • Mohammad Riazul Islam Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh

DOI:

https://doi.org/10.3329/jbas.v47i1.65129

Keywords:

Grammothele lineata, Endophyte, Epothilone B, Anti-Cancer drug, NaPDoS

Abstract

Jute (Corchorus olitorius) endophytic fungi Grammothele lineata SDL-CO-2015-1 was found to produce epothilone B, a well-reported anticancer compound. Both the genome and RNA-seq data of G. lineata revealed the presence of epothilone gene clusters. In silico mining using NaPDoS (Natural Product Domain Seeker) identified four genes matching with the epothilone gene cluster (Epo D). Epothilone was extracted from the extracellular extract of this fungus using XAD-16 resin. Different analytical techniques including thin layer chromatography (TLC), reverse phase high-performance liquid chromatography (RP-HPLC), and mass spectrometry (MS) were used to identify and characterize epothilone following its extraction. TLC bands were visible at 365 nm for both standard epothilone B and samples with identical Rf values. Extracted epothilone bands from the TLC plate were further purified using RP-HPLC. Using a linear gradient system with methanol and 0.1% formic acid, LC-MS spectroscopy was done with RP-HPLC purified fraction. The monoisotopic mass of epothilone (M+H+) = 508.1 Da was present in the mass chromatogram. The product ions of 508.1 Da were found with the m/z values at 320.2 Da, and 420.2 Da, the typical MS2 fragments for standard epothilone B. This finding suggests that the fungal endophyte, G. lineata can be a potential source of epothilone B.

J. Bangladesh Acad. Sci. 47(1); 91-99: June 2023

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Published

2023-06-27

How to Cite

Beg, M. A. ., Hannan, S. B. ., Rabeya, .-., Talukder, S. A. ., Uddin, A. ., Akter, S. ., Reza, R. N. ., Khan, H. ., & Islam, M. R. . (2023). Jute endophyte Grammothele lineata SDL-CO-2015-1 produces an anticancer compound epothilone B. Journal of Bangladesh Academy of Sciences, 47(1), 91–99. https://doi.org/10.3329/jbas.v47i1.65129

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Section

Research Articles