Mutation in neurospora crassa with leaf extract of citrus aurantifolia and their soluble protein content

Authors

  • Apurba Lal Ray Department of Botany, University of Dhaka, Dhaka
  • Mahbuba Akhter Jahan Department of Botany, University of Dhaka, Dhaka
  • Tahsina Rahim Department of Botany, University of Dhaka, Dhaka

DOI:

https://doi.org/10.3329/dujbs.v19i2.8958

Keywords:

Neurospora crassa, Mutation, Leaf extract, Soluble protein

Abstract

Leaf extract of Citrus aurantifolia exhibited remarkable inhibitor effect on the radial mycelial growth of Neurospora crassa. The extract also showed mutagenic effect and atleast six morphological mutants of the fungus were detected including albino (al 243), vigorous (vg 117), fluffy (fl 220), colonial (cl 232), conidial band (con. band 171) and dirty (dir 83). The mutants were used for estimation of soluble protein in comparison with the wild type (Ema). The soluble protein content increased to some extent in case of the mutants con. band 171 (192.86 μg/ml), cl 232 (188.57 μg/ml) and vg 117 (186.43 μg/ml) as compared to the wild type (182.14 μg/ml). On the other hand, the soluble protein content was remarkably decreased in case of the mutant al 243 (94.28 μg/ml), which was about 50% less than the control. This indicates that the leaf extract not only effect colony morphology but possesses profound effect on growth and metabolism of the fungus.

Key words: Neurospora crassa; Mutation; Leaf extract; Soluble protein

DOI: http://dx.doi.org/10.3329/dujbs.v19i2.8958

DUJBS 2010; 19(2): 151-155

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How to Cite

Ray, A. L., Jahan, M. A., & Rahim, T. (2011). Mutation in neurospora crassa with leaf extract of citrus aurantifolia and their soluble protein content. Dhaka University Journal of Biological Sciences, 19(2), 151–155. https://doi.org/10.3329/dujbs.v19i2.8958

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