Study of the Effect of Sodium Dodecyl Sulfate (SDS) and Acridine Orange on the Isolation of Plasmid and Antimicrobial Resistance Pattern of Clinical Isolates of <i>Klebsiella Sp</i>

  • Mohammad Shahriar Department of Pharmacy, The University of Asia Pacific, Dhanmondi, Dhaka
  • Shumaila Mawla Department of Pharmacy, The University of Asia Pacific, Dhanmondi, Dhaka
  • Mohiuddin Ahmed Bhuiyan Department of Pharmacy, The University of Asia Pacific, Dhanmondi, Dhaka
  • Mahboob Hossain Department of Microbiology, BRAC University, Mohakhali, Dhaka
Keywords: Klebsiella sp., plasmid, antimicrobial susceptibility, multidrug resistance, sodium dodecyl sulfate (SDS)

Abstract

For a six months period a total of 25 Klebisiella sp. samples collected from different diagnostic centers were tested for their antimicrobial susceptibility pattern against 16 antimicrobials. All isolates under study showed resistance against cephalexin, cephradine, ciporfloxacin, cloxacillin, erythromycin, oxacillin, rifampicin and tetracycline. Most of the isolates were resistant to amoxicillin (92%), vancomycin (96%), neomycin (84%) and chloramphenicol (76%). Least number of isolates showed resistance to tetracycline (36%), ceftriaxone (40%) and gentamycin (56%), respectively. Out of the 25 isolates, 7 multidrug resistant isolates were selected and they were grown in varying concentrations of Sodium Dodecyl Sulfate (SDS) in Luria broth and their plasmid profile was analysed. Distinct bands of plasmid were observed when the multidrug resistant strains of Klebsiella sp. were grown in 5% SDS and acridine orange (10μg/ml) in Luria broth. On being treated with 5% SDS and 5% SDS along with acridine orange, one isolate (isolate no. 3) developed sensitivity against ceftriaxone, ciprofloxacin, co-trimoxazole, gentamycin and neomycin.

Keywords: Klebsiella sp.; plasmid; antimicrobial susceptibility; multidrug resistance; sodium dodecyl sulfate (SDS)  

DOI: http://dx.doi.org/10.3329/diujst.v7i1.9646  

Daffodil International University Journal of Science and Technology Vol.7(1) 2012 38-43

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Shahriar, M., Mawla, S., Bhuiyan, M., & Hossain, M. (1). Study of the Effect of Sodium Dodecyl Sulfate (SDS) and Acridine Orange on the Isolation of Plasmid and Antimicrobial Resistance Pattern of Clinical Isolates of <i>Klebsiella Sp</i&gt;. Daffodil International University Journal of Science and Technology, 7(1), 38-43. https://doi.org/10.3329/diujst.v7i1.9646
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