Seroprevalence of anti-HEV and HEV RNA among volunteer blood donors and patients with Hepatitis B and C in Iran

Authors

  • Hossein Keyvani Department of virology, Iran University of Medical Sciences
  • Mahmood Shamsi Shamabadi Department of virology, Iran University of Medical Sciences
  • Saeed Najafifard Department of virology, Iran University of Medical Sciences
  • Bashir Hajibeigi Iranian Blood Transfusion Organization Research Center, Tehran, Iran
  • Farahnaz Fallahian Baqiyatallah Research Center for Gastroenterology and Liver Disease, Baqiyatallah University of Medical Sciences
  • Seyed-Moayed Alavian Baqiyatallah Research Center for Gastroenterology and Liver Disease, Baqiyatallah University of Medical Sciences

DOI:

https://doi.org/10.3329/blj.v1i1.2623

Keywords:

Hepatitis E virus, Seroprevalence, Transmission, Iran

Abstract

Aim: To assess seroprevalence of antibodies to hepatitis E virus (HEV) in healthy blood donors and hepatitis B, C patients.
Methods: 450 subjects consisted of 200 blood donors in Tehran blood transfusion center, 100 subjects with hepatitis C and 150 subjects with hepatitis B infection enrolled in this study. The A549 cell line was grown in mixed medium. Cells were infected with hepatitis E virus that was purified from stool sample of a patient confirmed for hepatitis E infection by reverse transcription-polymerase chain reaction (RT-PCR) method. Supernatant of infected cells was used as positive control in our RT- PCR assay.
Results: In a total of 450 subjects, 33 (7.3%) had positive anti-HEV by enzyme-linked immunosorbent assay (ELISA). Anti-HEV was seen in (9/200) 4.5%, (7/100) 7%, and (17/150) 11.3% of healthy blood donors, hepatitis C, and hepatitis B subjects, respectively. Difference between two groups was statistically significance (P = 0.028). Difference between frequency of anti-HEV in hepatitis B in relation to healthy blood donors was significant (P = 0.014).
Conclusions: HEV infection is more common in subjects with hepatitis B.

Keywords: Hepatitis E virus, Seroprevalence, Transmission, Iran  

doi: 10.3329/blj.v1i1.2623

Bangladesh Liver Journal Vol.1(1) 2009 p.34-37

 

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Author Biography

Seyed-Moayed Alavian, Baqiyatallah Research Center for Gastroenterology and Liver Disease, Baqiyatallah University of Medical Sciences

Department of Internal Medicine, Gastroenterology and Hepatology Baqiyatallah Research Center for
Gastroenterology and Liver Disease, Vanaq Square, Mola Sadra St. Tehran, Iran
E mail address: Alavian@thc.ir, Tell: +982188945186, Fax: +982188945188, PO BOX: 14155-3651

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How to Cite

Keyvani, H., Shamabadi, M. S., Najafifard, S., Hajibeigi, B., Fallahian, F., & Alavian, S.-M. (2009). Seroprevalence of anti-HEV and HEV RNA among volunteer blood donors and patients with Hepatitis B and C in Iran. Bangladesh Liver Journal, 1(1), 34–37. https://doi.org/10.3329/blj.v1i1.2623

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Original Articles