In vitro regeneration of three varieties of <i>Brassica campestris L.</i> grown in Bangladesh

Authors

  • B Goswami Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • MI Hoque Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh
  • S Khan Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • RH Sarker Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

DOI:

https://doi.org/10.3329/bjsir.v55i3.49391

Keywords:

Brassica campestris; Regeneration; Hypocotyl; In vitro flowering; Rooting

Abstract

A reproducible in vitro regeneration system was developed for three varieties of Brassica campestris L. namely Agrani, BINA Sarisha-10 and BINA Sarisha-6 using hypocotyl and cotyledonary leaf with petiole as explants. MS medium supplemented with 2.0 mg/l BAP and 0.2 mg/l IAA was found to be the best for the multiple shoot formation for all the three varieties. Among three varieties, BINA Sarisha-6 showed best response in terms of shoot regeneration as well as number of shoots per explant (9.0) using hypocotyls as explants. In case of Agrani and BINA Sarisha-10 highest number of shoot per explants were found 8.2 and 7.0, respectively. Interestingly in vitro regenerated shoots of BINA Sarisha-6 and BINA Sarisha-10 were produced in vitro flowers on shoot regeneration media. Best root induction in BINA Sarisha-6, Agrani and BINA Sarisha-10 was achieved on MS media supplemented with 0.5 mg/l IBA. After proper hardening, the in vitro regenerated plantlets were successfully transplanted into soil.

Bangladesh J. Sci. Ind. Res.55(3), 181-188, 2020

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Published

2020-09-24

How to Cite

Goswami, B., Hoque, M., Khan, S., & Sarker, R. (2020). In vitro regeneration of three varieties of <i>Brassica campestris L.</i> grown in Bangladesh. Bangladesh Journal of Scientific and Industrial Research, 55(3), 181–188. https://doi.org/10.3329/bjsir.v55i3.49391

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