<i>In vitro</i> Shoot Proliferation and Plant Regeneration of <i>Physalis minima</i> L. - a Perennial Medicinal Herb

Authors

  • Farhana Afroz Biological Research Division, BCSIR Laboratories, Dhaka
  • AKM Sayeed Hassan Biological Research Division, BCSIR Laboratories, Dhaka
  • Laila Shamroze Bari Biological Research Division, BCSIR Laboratories, Dhaka
  • Rebeka Sultana Biological Research Division, BCSIR Laboratories, Dhaka
  • Nadira Begum Biological Research Division, BCSIR Laboratories, Dhaka
  • Miskat Ara Akter Jahan Biological Research Division, BCSIR Laboratories, Dhaka
  • Rahima Khatun Biological Research Division, BCSIR Laboratories, Dhaka

DOI:

https://doi.org/10.3329/bjsir.v44i4.4597

Keywords:

Physalis minima, Medicinal plant, Shoot proliferation, Micropropagation, Regeneration

Abstract

The present study describes a protocol for high frequency plant regeneration of Physalis minima. Shoots were induced by culturing nodal segments and shoot tips from 15 day old seedlings. About 29 and 32 shoots were found to be induced from nodal segment and shoot tip explants, respectively, cultured on MS medium supplemented with 1.0 mg/l BAP. When shoots were subcultured on the fresh medium with same component as mentioned above, the shoots were elongated. Shoots rooted well when they were excised individually and implanted on half-strength MS medium with 0.3 mg/l NAA, where 98% shoots rooted within 12-15 days. In vitro grown plantlets with strong root system were successfully established in normal room temperature for seven days before transplanting in pots where they were reared for three weeks through successive acclimatization. The regenerated plants were successfully transferred to the soil with 90% survival rate.

Key words: Physalis minima; Medicinal plant; Shoot proliferation; Micropropagation; Regeneration

DOI: 10.3329/bjsir.v44i4.4597

Bangladesh J. Sci. Ind. Res. 44(4), 453-456, 2009

Downloads

Download data is not yet available.
Abstract
1184
PDF
1108

Downloads

Published

2010-03-12

How to Cite

Afroz, F., Hassan, A. S., Bari, L. S., Sultana, R., Begum, N., Jahan, M. A. A., & Khatun, R. (2010). <i>In vitro</i> Shoot Proliferation and Plant Regeneration of <i>Physalis minima</i> L. - a Perennial Medicinal Herb. Bangladesh Journal of Scientific and Industrial Research, 44(4), 453–456. https://doi.org/10.3329/bjsir.v44i4.4597

Issue

Vol. 44 No. 4 (2009)

Section

Articles

License

Bangladesh Council of Scientific and Industrial Research (BCSIR) holds the copyright to all contents published in Bangladesh Journal of Scientific and Industrial Research (BJSIR). A copyright transfer form should be signed by the author(s) and be returned to BJSIR.

The entire contents of the BJSIR are protected under Bangladesh Council of Scientific and Industrial Research (BCSIR) copyrights.

Creative Commons Licence
BJSIR is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC) Creative Commons Attribution-NonCommercial 4.0 International License which allows others remix, tweak, and build upon the articles non-commercially, and although their new works must also acknowledge and be non-commercial, they don’t have to license their derivative works on the same terms.