Plant Regeneration Through Nodal Explant Derived Callus in Wood Apple (<i>Aegle marmelos</i> L.)

Authors

  • Ranjoy Das Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi
  • M Faruk Hasan Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi
  • Harunar Rashid bDepartment of Botany, University of Rajshahi, Rajshahi
  • Motiur Rahman bDepartment of Botany, University of Rajshahi, Rajshahi

DOI:

https://doi.org/10.3329/bjsir.v44i4.4590

Keywords:

Plant regeneration, Callus induction, Nodal explant, Aegle marmelos

Abstract

This study reports on an improved protocol for callus induction and subsequent regeneration from nodal segment of wood apple (Aegle marmelos L.) Creamish friable competent callus was achieved from nodal segments on MS medium augmented with 4.0 mg1-1 2,4-D within two weeks of inoculation. The callus produced large number of shoots when cultured on MS medium fortified with 2.0 mgl-1 BAP+0.1 mgl-1 NAA within ten days of culture. In vitro raised shoots were rooted on half strength MS medium enriched with 1.0 mgl-1 IBA within fifteen days of culture. The rooted plantlets were successfully established with 80% survival.

Key words: Plant regeneration; Callus induction; Nodal explant; Aegle marmelos.

DOI: 10.3329/bjsir.v44i4.4590

Bangladesh J. Sci. Ind. Res. 44(4), 415-420, 2009

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How to Cite

Das, R., Hasan, M. F., Rashid, H., & Rahman, M. (2010). Plant Regeneration Through Nodal Explant Derived Callus in Wood Apple (<i>Aegle marmelos</i> L.). Bangladesh Journal of Scientific and Industrial Research, 44(4), 415–420. https://doi.org/10.3329/bjsir.v44i4.4590

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