Stimulating effect of fermented ginseng leaf saponin on the differentiation and mineralization of murine osteoblastic MC3T3-E1 cells
Keywords:β-glucosidase; Leaf saponin; MC3T3-E1; Differentiation; Mineralization
In this study, abundant ginseng leaf saponins were converted into minor ginsenosides that havemore pharmacological efficacy via fermentation process using recombinant β-glucosidase (bgp1). This fermented product was used to investigate the stimulatory effect on differentiation and mineralization of murine osteoblastic MC3T3-E1 cells. All major ginsenosides which areavailable in ginseng leaf were biotransformed into more pharmacologically active minor ginsenosides within a short time of incubation. The results showed that 100% of ginsenoside Rd, Rg1and Re were decomposed and transformed to Rg3, Rh1and Rg2, respectively within 03 (three) hours of incubation. Ginseng leaf saponin contains 17.1% Rg1, 44.9% Re, 10.8% Rd, 4.8% Rb1, 5.7% Rb2, 6.9% Rc,2.7% Rg2, and 6.8% F1 ginsenoside. But after fermentation, the products contain mostly pharmacological active minor ginsenosides including 42.2% Rg2, 13.7% Rg3, 8.8% Rh1, 4.9% F1 and 3.6% PPT ginsenosides. Moreover, we investigated and compared the effect of leaf saponins (LS) and fermented leaf saponins (FLS), on the differentiation and mineralization of pre-osteoblastic MC3T3-E1 cells. Treatment with FLS remarkably enhanced cell viability in a dose-dependent manner. FLS notably stimulated the ALP activity, Coll-I synthesis and mineralization ability of MC3T3-E1 cells. Based on the comparison between LS and FLS, it is clear that FLS has good effect on differentiation of osteoblastic MC3T3-E1 cells and bone formation. Therefore, bgp1-fermented ginseng leaf saponins could be a novel treatment for osteoporosis prevention.
Bangladesh J. Sci. Ind. Res.54(2), 147-154, 2019
How to Cite
Bangladesh Council of Scientific and Industrial Research (BCSIR) holds the copyright to all contents published in Bangladesh Journal of Scientific and Industrial Research (BJSIR). A copyright transfer form should be signed by the author(s) and be returned to BJSIR.
The entire contents of the BJSIR are protected under Bangladesh Council of Scientific and Industrial Research (BCSIR) copyrights.
BJSIR is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC) Creative Commons Attribution-NonCommercial 4.0 International License which allows others remix, tweak, and build upon the articles non-commercially, and although their new works must also acknowledge and be non-commercial, they dont have to license their derivative works on the same terms.