Antimicrobial Activity of Akanda (<i>Calotropis gigantea</i> L.) on Some Pathogenic Bacteria.
Keywords:Methanol extract, Akonda (Calotropis gigantea), Petroleum ether fraction, Chloroform fraction and Ethyl acetate fraction.
The antibacterial activity of methanol extract from the root bark of Akanda (Calotropis gigantea L.) and its petroleum ether, chloroform and ethyl acetate fractions were investigated. Both of methanol extract and its chloroform fraction showed activity against Sarcina lutea, Bacillus megaterium and Pseudomonas aeruginosa. Petroleum ether fraction showed activity against Bacillus subtilis and Shigella sonnei whereas ethyl acetate fraction showed activity against Pseudomonas aeruginosa and Escherichia coli at 20Ã¬g/disc, 30Ã¬g/disc and 40Ã¬g/disc doses. Among the tested materials, methanol extract and its chloroform fraction showed comparatively better results. Minimum inhibitory concentration (MIC) for methanol extract and each fraction were also determined by serial dilution technique.
Keywords: Methanol extract, Akonda (Calotropis gigantea), Petroleum ether fraction, Chloroform fraction and Ethyl acetate fraction. Â Â
DOI = 10.3329/bjsir.v43i3.1156
Bangladesh J. Sci. Ind. Res. 43(3), 397-404, 2008
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