Synergistic activity of methanolic extract of Thespesia populnea (malvaceae) flowers with oxytetracycline

Arthanari Saravana Kumar, K. Venkateshwaran, J. Vanitha, V.S. Saravanan, M. Ganesh, M. Vasudevan, T. Sivakumar

Abstract

The object of this study was to formulate new, cost effective anti-microbial combination for multidrug resistant diseases based on the synergistic activity of oxytetracycline with methanolic extract of Thespesia populnea (Malvaceae) a medicinal plant common in South India. The MIC of methanolic extracts in combination with oxytetracycline using 12 different both gram positive and gram negative bacteria was found to be around (62.5 µg/mL to 1000 µg/mL). The synergistic activity was verified using Kirby and Bauer techniques. 83.3% shows synergistic activity against all 12 different bacteria both gram positive and gram negative species. The highest synergism rate was attained against Shigella boydii (ATCC8700).

Keywords

Methanolic extract; Oxytetracycline; Synergistic activity; Thespesia populnea

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References

Betoni JEC, Mantouani RP, Barbosa LN, Stasi LCD, Fernander A. Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases. Mem Inst Oswaldo Cruz, Rio De Janerio. 2006; 101: 387-90.

Black JG. Microbiology: Principles and application. New York, Prentice Hall, 1996, p 260.

Chang S, Yahg M, Wen H, Chem J. Estimation of total flavonoid component in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002; 10: 178-82.

Evans WC. Treese and Evans pharmacognosy. London, Balliere, Tindall, 1996, p 388.

Ferreira MJU, Ascenso JR, Valdeira L, Duarte A, Frade JP, Freitas G. Evaluation of antiviral and antimicrobial activities of triterpenes isolated from Euphorbia segetalis. Nat Prod Res. 2003; 17: 375-80.

Gupta P, Murali P, Murali MV, Faridi MMA, Kaul PB, Ramachandran VC, Talwar V. Clinical profile of Klebsiella septicaemia in neonates. Indian J Paediatr. 1993; 60: 565-72.

Kumar AS, Mazumder, Saravanan VSA. Antihyperlipidemic activity of Camellia sinensis leaves in Triton-WR 1339 induced Albino rats. Phcog Mag. 2008; 4: 60-64.

Li S, Lo CY, Ho CT. Hydroxylated polymethoxy flavones and methylated flavonoids in sweet orange (Citrus sinensis) peel. J Agric Food Chem. 2006; 54: 4176-85.

Mastroeni P. Immunity to systemic salmonella infections. Curr Mol Med. 2002; 2: 393-406.

Nascimento GGF, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz J Microbiol. 2000; 31: 247-56.

Nazaruk J, Jakoniuk P. Flavonoid composition and antimicrobial activity of Crisium rivulare (jacq) flowers. J Ethnopharmacol. 2005; 102: 208-12.

Shirwaikarkumar A, Krishnan AV, Sreenivasan KK. Chemical investigation and antihepatotoxic activity of Thespesia populnea. Int J Pharmacog. 1995; 33: 305-10.



DOI: http://dx.doi.org/10.3329/bjp.v4i1.958

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Copyright (c) 2009 Arthanari Saravana Kumar, K. Venkateshwaran, J. Vanitha, V.S. Saravanan, M. Ganesh, M. Vasudevan, T. Sivakumar

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Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is a single-blinded peer-reviewed, video component, open access official publication of the Bangladesh Pharmacological Society (BDPS). eISSN: 1991-0088. For contact: Editor-In-Chief