Antidiarrheal and antispasmodic activities of Salvia officinalis are mediated through activation of K+ channels

Aslam Khan, Najeeb-ur- Rehman, Khalid M. AlKharfy, Anwarul-Hassan Gilani


This study was conducted to provide pharmacological basis for the medicinal use of Salvia officinalis in diarrhea. The crude extract of S. officinalis was studied using the in vivo and in vitro assays.  The crude extract inhibited castor oil-induced diarrhea in mice at the dose of 100 and 300 mg/kg. In isolated rabbit jejunum, it caused a dose-dependent (0.1-3 mg/mL) relaxation of spontaneous as well as low K+ (25 mM) and high K+ (80 mM)-induced contractions, being distinctly more potent on low K+. Pretreatment of tissue with 4-aminopyridine (1 mM) almost completely blocked the inhibitory effect of crude extract on low K+.  These results indicate that the crude extract of S. officinalis possesses antidiarrheal and antispasmodic activities mediated possibly through the dominant activation of voltage-dependent K+ channels and this study provides sound pharmacological basis for its medicinal use in diarrhea and gut spasm.


Antidiarrheal; Antispasmodic; Potassium channel; Salvia officinalis; Sage

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Akhondzadeh S, Noroozian M. Mohammadi M, Ohadinia S, Jamshidi AH, Khani M. Salvia officinalis extract in the treatment of patients with mild to moderate Alzheimer’s disease: A double blind, randomized and placebo-controlled trial. J Clin Pharm Ther. 2003; 28: 53–59.

Alarcon-Aguilar, FJ, Roman-Ramos R, Flores-Saenz JL, Aguirre-Garcia F.Investigation on the hypoglycaemic effects of extracts of four Mexican medicinal plants in normal and alloxan-diabetic mice. Phytoth Res. 2002; 16: 383–86.

Baricevic D, Sosa S, Della Loggia R, Tubaro A, Simonovska B, Krasna A, et al. Topical anti-inflammatory activity of Salvia officinalis L. leaves: The relevance of ursolic acid. J Ethnopharmacol. 2001; 75: 125-32.

Bashir S, Memon R, Gilani AH. Antispasmodic and antidiarrheal activities of Valeriana hardwickii rhizome are putatively mediated through calcium channel blockade. Evid Based Compl

Altern Med. 2011: 2011: 304960.

Cook NS. The pharmacology of potassium channels and their therapeutic potential. Trends Pharmacol Sci. 1988; 9; 21-28.

Duke JA, Bogenschutz-Godwin MJ, duCelliar J, Duke PAK. Handbook of medicinal herbs, 2nd ed. Boca Raton, CRC Press, 2002.

Eidi M, Eidi A, Zamanizadeh H. Effect of Salvia officinalis L. leaves on serum glucose and insulin in healthy and streptozotocin-induced diabetic rats. J Ethnopharmacol. 2005; 100: 310–13.

Empfield JR, Russell K, Trainor DA. Potassium channel openers: Therapeutic possibilities. Pharmaceutical News. 1995; 6: 23-27.

Fleming T. PDR for herbal medicines, 1st ed. Montvale, Medical Economics Company, 1998, p 1113.

Frank H, Puschmann A, Schusdziarra V, Allescher HD. Functional evidence for a glibenclamide-sensitive K+ channel in rat ileal smooth muscle. Eur J Pharmacol. 1994; 271: 379–86.

Gilani AH, Khan AU, Ghayur MN, Ali SF, Herzig JW. Antispasmodic effects of Rooibos tea (Aspalathus linearis) is mediated predominantly through K+-channel activation. Basic Clin Pharmacol Toxicol. 2006; 99: 365–73.

Gilani AH, Khan AU, Jabeen Q, Subhan F, Ghafar G. Antispasmodic and blood pressure lowering effects of Valeriana wallichii are mediated through K+ channel activation. J Ethnopharmacol. 2005; 100: 347-52.

Hamilton TC, Weir SW, Weston TH. Comparison of the effect of BRL34915 and verapamil on electrical and mechanical activity in rat portal vein. Brit J Pharmacol. 1986; 88: 103-11.

Imanshahidi M, Hosseinzadeh H. The pharmacological effects of Salvia species on the central nervous system. Phytother Res. 2006; 20: 427-37

Iwao I, Terada Y. On the mechanism of diarrhea due to castor oil. Jap J Pharmacol. 1962; 12: 137-45.

Jalsenjak, V, Peljnajk, S, Kustrak D. Microcapsules of sage oil, essential oils content and antimicrobial activity. Pharmazie. 1987; 42: 419–20.

Jebunnessa, Uddin SB, Mahbub-Uz-Zaman M, Akhtar R, Ahmed NU. Antidiarrheal activity of ethanolic bark extract of Mitragyna diversifolia. Bangl J Pharmacol. 2009; 4: 144-46.

Kishii KI, Morimoto T, Nakajima N, Yamazaki K, Tsujitani M, Takayanagi I. Effect of LP-805, a novel vasorelaxant agent, a potassium channel opener on rat thoracic aorta. Gen Pharmacol. 1992; 23: 347-53.

Lenz T, Wagner G. Potential role of potassium channel openers for the treatment of cardiovascular disease. In: Hypertension: Pathophysiology, diagnosis and management. Laragh JH, Brenner BM (eds). New York, Raven Press, 1995, pp 2953-68.

Limaa CF, Andradeb PB, Seabrab RM, Fernandes-Ferreiraa M, Pereira-Wilson C, The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats. J Ethnopharmacol. 2005; 97; 383–89.

Lu Y,Foo LY. Salvianolic acid L, a potent phenolic antioxidant from Salvia officinalis. Tetrahedron Lett. 2001; 42; 8223–25.

Mannhold R. KATP channel openers: structure-activity relationships and therapeutic potential. Med Res Rev. 2004; 24; 213-66.

National Research Council, Guide for the Care and Use of Laboratory Animals. Washington, National Academy Press, 1996, pp 1-5.

Newall C, Anderson LA, Phillipson JD. In: Herbal medicines: A guide for health-care professionals. Newall CA, Anderson LA, Phillipson JD (eds). The Pharmaceutical Press, London, 1996.

Okabe K, Kitamura K, Kuriyama H. Feature of 4-aminopyridine sensitive outward current observed in single smooth muscle cells from the rabbit pulmonary artery. Pfluegers Arch. 1987; 409: 561-68.

Qnais EY, Abu-Dieyeh M, Abdulla FA, Abdalla SS. The antinociceptive and anti-inflammatory effects of Salvia officinalis leaf aqueous and butanol extracts. Pharm Biol. 2010; 48: 1149-56.

Quest U. Potassium channel openers: Pharmacological and clinical aspects. Fund Clin Pharmacol. 1992; 6: 279-93.

Shah AJ, Zaidi MA, Hamidullah SH, Gilani AH. Antidiarrheal and antispasmodic activities of Vincetoxicum stocksii are mediated through calcium channel blockade. Bangladesh J Pharmacol. 2011; 6: 46-50.

Shah AJ, Begum S, Hassan SI, Ali SN, Siddiqui BS, Gilani AH. Pharmacological basis for the medicinal use of Psidium guajava leave in hyperactive gut disorders Bangladesh J Pharmacol. 2011; 6: 100-105.

Tada M, Okuno K, Chiba K, Ohnishi E, Yoshii T. Antiviral diterpenes from Salvia officinalis. Phytochem. 1994; 35: 539-41.

Vogalis F. Potassium channels in gastrointestinal smooth muscle. J Auton Pharmacol. 2000; 20; 207-19.

Vujosević M, Blagojević J. Antimutagenic effects of extracts from sage (Salvia officinalis) in mammalian system in vivo. Acta Vet Hung. 2004; 52: 439-43.

Wang M, Li J, Rangarajan M, Shao Y, LaVoie EJ, Hang T-Ch, Ho Ch-T. Anti-oxidative phenolic compounds from sage (Salvia officinalis). J Agri Food Chem. 1998; 46: 4869-73.

Williamson EM, Okpako DT, Evans FJ. Selection, preparation and pharmacological evaluation of plant material. Chichester, John Wiley & Sons, 1998, pp 15-23.


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