Hepatoprotective effect of Pluchea pteropoda extract against CCl4-induced hepatotoxicity
Keywords:
Carbon tetrachloride, Hepatoprotective, Pluchea pteropodaAbstract
This study aimed to evaluate the hepatoprotective effects of P. pteropoda extract against carbon tetrachloride (CCl4)-induced hepatotoxicity. The extraction condition was due to ethanol 50%, a solid-to-solvent ratio of 1:10, extraction temperature of 60°C, and extraction time of 4 hours. Phytochemical analysis indicated that total polyphenol and flavonoid contents are 76.5 ± 5.2 mg GAE/g DW and 56.8 ± 2.6 mg QE/g DW, respectively. Notably, pretreatment with the extract increased the viability of hepatic cells up to 71.5% at 200 µg/ml. Moreover, oral administration of the extract at 200 mg/kg reduced aspartate transaminase and alanine transaminase elevation levels by 65% and 59% as compared to CCl4 treatment alone. The extract also improved body weight, maintained survival, restored hepatic GSH level, and reduced lipid peroxidation. These findings demonstrate that P. pteropoda exerts hepatoprotective activity against CCl4-induced hepatotoxicity through antioxidant-mediated mechanisms.
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References
Alkinani KB, Ali EMM, Al-Shaikh TM, Awlia Khan JA, Al-Naomasi TM, Ali SS, Abduljawad AA, Mosa OF, Zafar TA. Hepatoprotective effects of (-) epicatechin in CCl4-induced toxicity model are mediated via modulation of oxidative stress markers in rats. Evid Based Complement Alternat Med. 2021; 2021: 4655150.
Bui TP, Ha QT, Pham HH, Nguyen DH, Bui AT, Dao DBN, Tran NMA, Le GH, Tran TTL. Xanthine oxidase inhibitory activity and uric acid-lowering effect in mice of the extract from Pluchea pteropoda Hemsl. Hong Bang Int Univ J Sci. 2026; 39: 59-66.
Chandra S, Khan S, Avula B, Lata H, Yang MH, ElSohly MA, Khan IA. Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops: A comparative study. Evid Based Complement Alternat Med. 2014; 2014: 253875.
Colak E, Ustuner MC, Tekin N, Colak E, Burukoglu D, Degirmenci I, Gunes HV. The hepatocurative effects of Cynara scolymus L. leaf extract on carbon tetrachloride-induced oxidative stress and hepatic injury in rats. Springerplus 2016; 5: 216.
Devarbhavi H, Asrani SK, Arab JP, Nartey YA, Pose E, Kamath PS. Global burden of liver disease: 2023 update. J Hepatol. 2023; 79: 516-37.
Di Paola R, Modafferi S, Siracusa R, Cordaro M, D’Amico R, Ontario ML, Interdonato L, Salinaro AT, Fusco R, Impellizzeri D, Calabrese V, Cuzzocrea S. S-acetyl-glutathione attenuates carbon tetrachloride-induced liver injury by modulating oxidative imbalance and inflammation. Int J Mol Sci. 2022; 23: 4429.
El-Toumy SA, Ahmed SA, Kamel EM. Phenolic constituents, hepatoprotective and cytotoxic activities of Pluchea dioscoridis. Int J Appl Res Nat Prod. 2014; 7: 1-10.
Gonzalez LT, Minsky NW, Espinosa LE, Aranda RS, Meseguer JP, Perez PC. In vitro assessment of hepatoprotective agents against damage induced by acetaminophen and CCl4. BMC Complement Altern Med. 2017; 17: 39.
Joshi BC, Prakash A, Kalia AN. Hepatoprotective potential of antioxidant potent fraction from Urtica dioica Linn. (whole plant) in CCl4 challenged rats. Toxicol Rep. 2015; 2: 1101-10.
Karamalakova YD, Nikolova GD, Georgiev TK, Gadjeva VG, Tolekova AN. Hepatoprotective properties of Curcuma longa L. extract in bleomycin-induced chronic hepatotoxicity. Drug Discov Ther. 2019; 13: 9-16.
Katarey D, Verma S. Drug-induced liver injury. Clin Med. 2016; 16: s104-09.
Liao Y, Lv F, Quan T, Wang C, Li J. Flavonoids in natural products for the therapy of liver diseases: Progress and future opportunities. Front Pharmacol. 2024; 15: 1485065.
Nguyet NTA, Thanh TD, Phat NT, Tai LM, Chi LTK. Study on isolation of some phenolic compounds from the root of Pluchea pteropoda Hemsl. TNU J Sci Technol. 2024; 229: 117-23.
Ouassou H, Bouhrim M, Daoudi NE, Mekhfi H, Ziyyat A, Legssyer A, Aziz M, Bnouham M. Evaluation of hepatoprotective activity of Caralluma europaea stem extract against CCl4-induced hepatic damage in Wistar rats. Adv Pharmacol Pharm Sci. 2021; 2021: 8883040.
Pham HH, Bui PT, Bui TA, Dao NDB, Than TM. Phytochemical composition, antioxidant, and acetylcholinesterase inhibitory activities of Pluchea pteropoda extract. Trop J Nat Prod Res. 2025; 9: 6203-08.
Phung TH, Nguyen NPT, Nguyen NT, Phan TD, Nguyen PD, Do TK, Nguyen DD, Nguyen THP. The investigation of morphological, anatomical and antibacterial activities of Pluchea indica (L.) Less. and Pluchea pteropoda Hemsl. Can Tho Univ J Sci. 2020; 58: 132-39.
Rasool M, Iqbal J, Malik A, Ramzan HS, Qureshi MS, Asif M, Qazi MH, Kamal MA, Chaudhary AG, Al-Qahtani MH, Gan SH, Karim S. Hepatoprotective effects of Silybum marianum (silymarin) and Glycyrrhiza glabra (glycyrrhizin) in combination: A possible synergy. Evid Based Complement Alternat Med. 2014; 2014: 641597.
Sanchez-Moreno C. Review: Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Technol Int. 2002; 8: 121-37.
Sen T, Basu A, Ray RN, Nag Chaudhuri AK. Hepatoprotective effects of Pluchea indica extract in experimental acute liver damage in rodents. Phytother Res. 1993; 7: 352-5.
Sherif IO, Al-Shaalan NH. Hepatoprotective effect of Ginkgo biloba extract against methotrexate-induced hepatotoxicity via targeting STAT3/miRNA-21 axis. Drug Chem Toxicol. 2022; 45: 1723-31.
The N, Tuan AL, Thu TDT, Luyen ND, Tran TT. Essential oils of the Asteraceae plants Blumea riparia DC. and Pluchea pteropoda Hemsl. ex Hemsl. growing in Vietnam. Nat Prod Commun. 2022; 17: 1-6.
Tithi TI, Tahsin MR, Anjum J, Zaman TS, Aktar F, Bahar NB, Tasnim S, Sultana A, Jahan I, Afrin SS, Akter T, Sen P, Koly FJ, Reza MS, Chowdhury JA, Kabir S, Chowdhury AA, Amran MS. An in vivo and in silico evaluation of the hepatoprotective potential of Gynura procumbens: A promising agent for combating hepatotoxicity. PLoS One. 2023; 18: e0291125.
Tran NT, Thu PNT, Minh HPT, Ngo QMT. In vivo acute oral toxicity and anti-inflammatory effects of Pluchea pteropoda ethanol extract. Vietnam Med J. 2024; 538: 60-4.
Ugwu CE, Suru SM. Medicinal plants with hepatoprotective potentials against carbon tetrachloride-induced toxicity: A review. Egypt Liver J. 2021; 11: 88.
Ullah H, Khan A, Baig MW, Ullah N, Ahmed N, Tipu MK, Ali H, Khan S. Poncirin attenuates CCL4-induced liver injury through inhibition of oxidative stress and inflammatory cytokines in mice. BMC Complement Med Ther. 2020; 20: 115.
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