In vitro antiproliferative activity of Dracaena fragrans ‘Lemon Lime’ on MCF-7 breast cancer cell lines
Keywords:
Anticancer activity; Antioxidant activity; DPPH assay; Dracaena fragrans Lemon lime; MCF-7 cell line; Phytochemical screeningAbstract
This study aimed to evaluate the in vitro antiproliferative activity of leaves of Dracaena fragrans ‘Lemon Lime’ on MCF-7 breast cancer cell lines. Phytoconstituents were extracted from leaves, and their total flavonoid contents were measured. Cell viability of cell lines was assessed by the MTT assay. The extract contained alkaloids, tannins, phenolics, and flavonoids, with a total flavonoid content of 161.7 mg QE/g. The IC₅₀ of the extract on cell lines was 64.9 µg/mL. These findings indicate that D. fragrans ‘Lemon Lime’ possesses notable antioxidant and antiproliferative activity.
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References
Abotaleb M, Samuel SM, Varghese E, Varghese S, Kubatka P, Liskova A, Busselberg D. Flavonoids in cancer and apoptosis. Cancers 2018; 11: 28.
Abubakar AR, Haque M. Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes. J Pharm Bioallied Sci. 2020; 12: 01-10.
Adedapo AA, Jimoh FO, Afolayan AJ, Masika PJ. Antioxidant activities and phenolic contents of the methanol extracts of the stems of Acokanthera oppositifolia and Adenia gummifera. BMC Complement Altern Med. 2008; 8: 54.
Agarwal A, Kumar A, Singh BK, Trivedi N, Jha KK. A review on extraction and phytochemical screening methods. Res Pharm Health Sci. 2016; 2: 130-37.
Apak R, Gorinstein S, Bohm V, Schaich KM, Ozyurek M, Guclu K. Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report). Pure Appl Chem. 2013; 85: 957-98.
Bellamakondi PK, Godavarthi A, Ibrahim M, Kulkarni S, Naik RM, Maradam S. In vitro cytotoxicity of Caralluma species by MTT and trypan blue dye exclusion. Asian J Pharm Clin Res. 2014; 7: 17-19.
Borzabadi Farahani F, Rahimi J, Khoramipour S, Dehghanifard E, Alimohammadi M. Environmental modulation enhances benzene phytoremediation by Aglaonema and Dracaena in indoor settings. Sci Rep. 2025; 15: 31559.
Brand-Williams W, Cuvelier ME, Berset CL. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol. 1995; 28: 25-30.
Brglez Mojzer E, Knez Hrncic M, Skerget M, Knez Z, Bren U. Polyphenols: Extraction methods, antioxidative action, bioavailability and anticarcinogenic effects. Molecules. 2016; 21: 901.
Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 2010; 15: 7313-52.
Dhyani P, Quispe C, Sharma E, Bahukhandi A, Sati P, Attri DC, Szopa A, Sharifi-Rad J, Docea AO, Mardare I, Calina D. Anticancer potential of alkaloids: A key emphasis to colchicine, vinblastine, vincristine, vindesine, vinorelbine and vincamine. Cancer Cell Int. 2022; 22: 206.
Gidamo GH. Antioxidant activity and mineral content of Impatiens tinctoria A. Rich (Ensosila) tuber, an Ethiopian medicinal plant. Sci Rep. 2023; 13: 14998.
Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. J Nutr Biochem. 2002; 13: 572-84.
Kopustinskiene DM, Jakstas V, Savickas A, Bernatoniene J. Flavonoids as anticancer agents. Nutrients 2020; 12: 457.
Kumar R, Verma V, Thakur M, Singh G, Bhargava B. A systematic review on mitigation of common indoor air pollutants using plant-based methods: A phytoremediation approach. Air Qual Atmos Health. 2023; 16: 1501-27.
Majid N, Nissar S, Raja WY, Nawchoo IA, Bhat ZA. Pharmacognostic standardization of Aralia cachemirica: A comparative study. Future J Pharm Sci. 2021; 7: 33.
Manikyam HK, Joshi SK, Vakadi S, Patil SB. Anticancer acti-vity of novel terpenoid, saponin of Psidium guajavaon MCF-7 cancer cell line using DAPI and MTT assays. Afr J Pharm Pharmacol. 2021; 15: 206-11.
Peterson G, Jones T, Rispoli D, Haddadi S, Niri V. Investigation of simultaneous volatile organic compound removal by indoor plants using solid phase microextraction-gas chromatography-mass spectrometry. RSC Adv. 2023; 13: 26896-06.
Qin Y, Qian C, Li W, Wang Q, Sheng Q, Chen Z, Zhang W, Li W, Ge G, Yan Z, Geng D. Oxidative stress: Molecular mechanisms, diseases, and therapeutic targets. Med Comm. 2026; 7: e70600.
Sawant VJ, Bamane SR, Shejwal RV, Patil SB. Comparison of drug delivery potentials of surface functionalized cobalt and zinc ferrite nanohybrids for curcumin in to MCF-7 breast cancer cells. J Magn Magn Mater. 2016; 417: 222-29.
Shraim AM, Ahmed TA, Rahman MM, Hijji YM. Determi-nation of total flavonoid content by aluminum chloride assay: A critical evaluation. LWT. 2021; 150: 111932.
Sun J, Liu JN, Fan B, Chen XN, Pang DR, Zheng J, Zhang Q, Zhao YF, Xiao W, Tu PF, Song YL. Phenolic constituents, pharmacological activities, quality control, and metabolism of Dracaena species: A review. J Ethnopharmacol. 2019; 244: 112138.
Talib WH, Alsalahat I, Daoud S, Abutayeh RF, Mahmod AI. Plant-derived natural products in cancer research: extraction, mechanism of action, and drug formulation. Molecules 2020; 25: 5319.
Teodor ED, Ungureanu O, Gatea F, Radu GL. The potential of flavonoids and tannins from medicinal plants as anticancer agents. Anti-Cancer Agents Med Chem. 2020; 20: 2216-27.
Wagner H, Ulrich-Merzenich G. Synergy research: Approaching a new generation of phytopharmaceuticals. Phytomedicine 2009; 16: 97-110.
Wendlocha D, Kubina R, Krzykawski K, Mielczarek-Palacz A. Selected flavonols targeting cell death pathways in cancer therapy: The latest achievements in research on apoptosis, autophagy, necroptosis, pyroptosis, ferroptosis, and cuproptosis. Nutrients 2024; 16: 1201.
Wu Z, Xia F, Lin R. Global burden of cancer and associated risk factors in 204countries and territories, 1980–2021: A systematic analysis for the GBD 2021. J Hematol Oncol. 2024; 17: 119.
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