Analysis of essential oil of eaglewood tree (Aquilaria agallocha Roxb.) by gas chromatography mass spectrometry


  • Mohammad Nazrul Islam Bhuiyan BCSIR Laboratories, P.O. Chittagong Cantonment, Chittagong 4220
  • Jaripa Begum BCSIR Laboratories, P.O. Chittagong Cantonment, Chittagong 4220
  • Md. Nurul Huda Bhuiyan BCSIR Laboratories, P.O. Chittagong Cantonment, Chittagong 4220



Aquilaria agallocha, Eaglewood, Essential oil, GC-MS, Octacosane


The study was carried out to find out the differences in composition of oils obtained from healthy, naturally infected and artificially screws wounds eaglewood (Aquilaria agallocha Roxb.) using gas chromatography mass spectrometry (GC-MS) analysis. Natural healthy plants agar contained octacosane (19.83%), naphthalene, 1,2,3,5,6,7,8,8a-octahydro-1,8a-dimethyl-7-(1-methylethenyl)-, [1R-(1.alpha.,7.beta.,8a.alpha.)]- (12.67%), 5-isobutyramido-2-methyl pyrimidine (13.52%), caryophyllene oxide (11.25%) and (.+-.)-cadinene (5.46%). Natural infected plants agar (super agar) contained cycloheptane, 4-methylene-1-methyl-2-(2-methyl-1-propen-1-yl)-1-vinyl- (46.17%), caryophyllene oxide (33.00%) and 7-Isopropenyl-4a-methyl-1-methylenedecahydronaphthalene (20.83%). Artificially screw injected plants agar contained diisooctyl phthalate (71.97%), 1H-Cycloprop[e]azulen-4-ol, decahydro-1,1,4,7-tetramethyl-, [1ar-(1a.alpha.,4.beta.,4a.beta., 7.alpha., 7a.beta., 7b.alpha.)]- (9.16%), hexadecanoic acid (7.05%), naphthalene, 1,2,3,5,6,7,8,8a-octahydro-1,8a-dimethyl-7-(1-methylethenyl)-, [1R-(1.alpha.,7.beta.,8a.alpha.)]- (6.45%) and aristolene (5.36%). This study showed a marked difference in the oil compositions among the treatments with regards to their quality.


Download data is not yet available.
2773 Read

Author Biography

Mohammad Nazrul Islam Bhuiyan, BCSIR Laboratories, P.O. Chittagong Cantonment, Chittagong 4220

Scientific officer


Anonymous. The wealth of India: Raw materials. Vol 1. New Delhi, Publication and Information Directorate, CSIR, 1948, pp 88-90.

Beniwal BS. Silvical characteristics of Aquilaria agallocha Roxb. Indian Forest. 1989; 115: 17-21.

Bhandari P, Pant R, Rastogi RP. Aquillochin: A coumarinolignan from Aquilaria agallocha. Phytochemistry 1982; 21: 2147-49.

Bhattacharyya B, Datta A, Baruah HK. On the formation and development of agaru in Aquilaria agallocha. Sci Cult. 1952; 18: 240- 41.

Bose SR. The nature of 'Agaru' formation. Sci Cult. 1938; 4: 89-91.

Clevenger JF. Apparatus for determination of volatile oil. J Amer Pharm Assoc. 1928; 17: 346.

Gibson IAS. The role of fungi in the origin of oleoresin deposits (Agaru) in the wood of Aquillaria agallocha (Roxb.). Bano Biggyn Patrika. 1977; 6: 16-26.

Ishihara M, Tsuneya T, Uneyama K. Fragrant sesquiterpenes from agarwood. Phytochemistry 1993; 33: 1147-55.

Ishihara M, Tsuneya T, Uneyama K. Guaiene sesquiterpenes from agarwood. Phytochemistry 1991; 30: 3343-47.

Jalaluddin M. A useful pathological condition agarwood. Econ. Bot. 1977; 31: 222-24.

Maheshwari ML, Jain TC, Bates RB, Bhattacharyya SC. Structure and absolute configuration of ?-agarofuran, ?-agarofuran and dihydroagarofuran. Tetrahedron. 1963; 19: 1079-90.

Mitra J, Gogoi P. Fungi associated with the diseased wood (agarwood/agaru) of Aquilaria agallocha Roxb. (Family: Thymelaeaceae) grown in Assam. Proceedings of seminar on scope and dimension of agarplantation in NE region Ahmed M, Gogoi P, Ahmed GU, Hojai AATMA (eds). India, 2001, pp 61-69.

Nagashima T, Kawasaki I, Yoshida T, Nakanishi T, Yoneda K, Miura I. New Sesquiterpenoids from agarwood. Paper IXth International essential oil congress. Singapore, 1983, pp 12-16.

Nakanishi T, Yamagata E, Yoneda K, Nagashima T, Kawasaki I, Yoshida T, Mori H, Miura I. Three fragrant sesquiterpenes of agarwood. Phytochemistry 1984; 22: 2066-67.

Okugawa H, Ueda R, Matsumoto K, Kawanishi K, Kato A. Effect of Agarwood on the central nervous system in mice. Planta Medica. 1993; 59: 32-36.

Paknikar SK, Naik CG. Stereochemistry of dihydroagarofurans and evidence in support of the structure of 4,11- epoxy-cis-eudesmane. Tet Lett. 1975; 15: 1293-94.

Pant P, Rastogi RP. Agarol, a new sesquiterpene from Aquilaria agallocha. Phytochemistry 1980; 19: 1869-70.

Qi Shu-Yuan, Lu Bi-Yau, Zhu Liang-Feng, Li Bao-Ling. Formation of oxoagarospirol in Aquilaria sinensis. Plant Physiol Commun. 1992; 28: 336-39.

Tamuli P, Boruah P. Changes in amino acids in agarwood plant under pathological condition. Geobios. 2002a; 29: 241-43.

Tamuli P, Boruah P. Vesicular-arbuscular mycorrhizal (VAM) association of agarwood tree in Jorhat district of the Brahmaputra Valley. Indian Forest. 2002b; 128: 991-94.

Tamuli P, Boruah P, Nath SC. Essential oil of eaglewood tree: A product of pathogenisis. J Essent Oil Res. 2005; 17: 601-04.

Tamuli P, Boruah P, Nath SC, Samanta R. Fungi from diseased agarwood tree (Aquilaria agallocha Roxb.): Two new records. Adv Forest Res India 2000a; 22: 182-87.

Tamuli P, Boruah P, Nath SC, Samanta R. Mycofloral study on the phyllosphere and soil of agarwood tree plantation. U.G.C. Sponsored seminar on conservation of biodiversity, Assam, 2000b; p 5.

Thomas AF, Ozainne M. The stereochemistry of the dihydroagarofurans. Tet Lett. 1976; 20: 1717-18.

Varma KR, Maheshwari ML, Bhattacharyya SC. The constitution of agarospisol, a sesquiterpenoid with a new skeleton. Tetrahedron 1965; 21: 115-38.

Venkataramanan MN, Borthakur R, Singh HD. Occurrence of endotrophic myccorhizal fungus in agarwood plant Aquilaria agallocha Roxb. Curr Sci. 1985; 54: 928.

Additional Files



How to Cite

Bhuiyan, M. N. I., J. Begum, and M. N. H. Bhuiyan. “Analysis of Essential Oil of Eaglewood Tree (Aquilaria Agallocha Roxb.) by Gas Chromatography Mass Spectrometry”. Bangladesh Journal of Pharmacology, vol. 4, no. 1, Aug. 2008, pp. 24-28, doi:10.3329/bjp.v4i1.851.



Research Articles